药学研究2026，Vol.45Issue(4)：388-394,7.DOI:10.13506/j.cnki.jpr.2026.04.005
整合转录组学与分子对接预测次大风子素抗结直肠癌的多靶点机制
Integrating transcriptomics with molecular docking to predict the multi-target mechanism of hydnocarpin D against colorectal cancer
摘要
Abstract
Objective To investigate the inhibitory effects of hydnocarpin D on colorectal cancer cells and elucidate its potential mechanisms.Methods Cell viability of HCT116,HepG2,and SGC7901 cells was assessed using the CCK-8 assay.RNA sequencing of hydnocarpin D-treated HCT116 cells was performed to identify differentially expressed genes(DEGs),followed by GO/KEGG enrichment and protein-protein interaction(PPI)analyses.Molecular docking was conducted to evaluate the binding affinity between hydnocarpin D and candidate targets.Results Hydnocarpin D inhibited cell proliferation in a dose-dependent manner,with HCT116 cells being the most sensitive(IC50=13.02 μmol·L-1).A total of 2 599 DEGs were identified,mainly enriched in the p53 signaling pathway,PI3K-Akt pathway,and ribosome biogenesis.Docking analysis revealed stable interactions between hydnocarpin D and KRAS,caspase-9,GSK3β.Conclusion Hydnocarpin D may exert anti-colorectal cancer effects through multi-target modulation of tumor-related signaling pathways,supporting its potential as a candidate anticancer compound.关键词
次大风子素/结直肠癌/转录组学/p53 信号通路/分子对接Key words
Hydnocarpin D/Colorectal cancer/Transcriptomics/p53 signaling/Molecular docking分类
医药卫生引用本文复制引用
张玫倩,梁艳妮,张艺萱,徐胜,辛乔艺,孙驭,周静..整合转录组学与分子对接预测次大风子素抗结直肠癌的多靶点机制[J].药学研究,2026,45(4):388-394,7.基金项目
陕西省教育厅青年创新团队项目(No.24JP047) （No.24JP047）
陕西省科技厅科技计划项目(No.2024JC-YBQN-0945) （No.2024JC-YBQN-0945）
陕西省教育厅科学研究计划项目(No.23JK0405) （No.23JK0405）
陕西省大学生创新训练计划项目(No.S202410716057) （No.S202410716057）
