药学研究2026，Vol.45Issue(4)：402-410,9.DOI:10.13506/j.cnki.jpr.2026.04.007
柳叶沙棘黄酮的绿色提取及抗氧化活性分析
Green extraction and antioxidant activity analysis of Hippophae rhamnoides flavonoids
摘要
Abstract
Objective To achieve green extraction of flavonoids from Hippophae rhamnoides,identify the flavonoid components,and analyze their antioxidant activity.Methods An ultrasound-assisted deep eutectic solvent(DES)system was employed.The optimal DES was screened,and microstructural changes in the samples were observed.The extraction process was optimized through single-factor experiments and response surface methodology.The flavonoid composition was analyzed,and antioxidant capacity was evaluated.Results Choline chloride—lactic acid was identified as the optimal DES.The optimized extraction parameters were a liquid-solid ratio of 40∶1(mL∶g),a temperature of 55℃,and a time of 33 min,yielding a flavonoid content of(77.12±0.12)mg·g-1.High-performance liquid chromatography identified four flavonoids:ru-tin,isoquercitrin,quercitrin,and quercetin.The extract exhibited antioxidant activity with IC50 values for the antioxidant scav-enging capacity against ABTS+and PTIO were 0.39 mg·mL-1 and 1.17 mg·mL-1,respectively.Conclusion The ultrasound-assisted deep eutectic solvent method shows promising potential for the extraction of flavonoids from Hippophae rhamnoides.关键词
沙棘黄酮/低共熔溶剂/工艺优化/成分鉴定/抗氧化活性Key words
Hippophae flavonoids/Deep eutectic solvent/Process optimization/Compound identification/Antioxidant activity分类
医药卫生引用本文复制引用
王谦,董洋宏,张莹..柳叶沙棘黄酮的绿色提取及抗氧化活性分析[J].药学研究,2026,45(4):402-410,9.基金项目
黑龙江省自然科学基金项目(No.LH2024C034) （No.LH2024C034）
