药学研究2026，Vol.45Issue(4)：411-415,5.DOI:10.13506/j.cnki.jpr.2026.04.008
泡桐果皮中碳取代香叶基二氢黄酮含量的检测方法与提取工艺研究
Study on the content determination and extraction process of C-geranylflavanones from Paulownia pericarp
摘要
Abstract
Objective To establish a content determination method of total C-geranylflavanones(TGFs)in Paulownia peel and optimize its extraction process.Methods A UV spectrophotometric method for the determination of TGFs in Pau-lownia peel was established.Furthermore,its extraction process of was optimized by L9(34)orthogonal test,with the extrac-tion rate and purity of TGFs as the evaluation indicators.Besides,the ethanol concentration,extraction time,extraction times and ethanol dosage were used as the inspecting factors.Results The extraction of Paulownia peel revealed the similar UV spectrum with a maximum absorption at λ 295 nm to that of diplacone,a reference substance obtained from Paulownia peel,and a good linear range was shown within the range of 0.005 to 0.038 mg·mL-1(R2=0.9994).The optimum extraction process was determined as follows:80％ethanol was used to extract for 1 times,0.5 h for each time,and the solid-liquid rati-o was 1∶10,the extraction rate and purity of TGFs were 26.60％and 62.70％respectively.By this extraction process,the content of TGFs in Paulownia peel harvested in May and Sep.was higher than those harvested in other months.Conclusion Content detection and optimized extraction methods of TGFs were all stable,reliable,and easily to obtain TGFs from Pau-lownia peel.The suitable harvested time for Paulownia fruits is in Sep.every year.关键词
泡桐果皮/碳取代香叶基二氢黄酮/紫外分光光度法/正交试验/提取优化/采收时间Key words
Paulownia peel/C-geranylflavanones/UV spectrophotometry/Orthogonal experiment/Optimization of ex-traction/Harvested time分类
医药卫生引用本文复制引用
谷文宇,王文君,柴君恒,贾献慧,肖成梅,唐文照..泡桐果皮中碳取代香叶基二氢黄酮含量的检测方法与提取工艺研究[J].药学研究,2026,45(4):411-415,5.基金项目
国家中医药管理局科技司-山东省卫生健康委员会共建中医药科技项目(No.GZY-KJS-SD-2023-087) （No.GZY-KJS-SD-2023-087）
