生物加工过程2026，Vol.24Issue(2)：127-134,8.DOI:10.3969/j.issn.1672-3678.2026.02.002
基于常压室温等离子体(ARTP)诱变的胞嘧啶高产菌株筛选及其发酵条件优化
Screening of high cytosine-producing strains by atmospheric and room temperature plasma(ARTP)mutagenesis and optimization of fermentation conditions
摘要
Abstract
Cytosine is widely applied in pharmaceutical and agricultural fields.Traditional chemical synthesis methods are often accompanied with high environmental pollution,exorbitant costs,and complex purification processes,so microbial fermentation has emerged as a viable alternative due to its environmental friendliness and sustainability.In this study,a high-yielding mutant strain(E.coli Cyto-85)was developed from the parental strain E.coli through atmospheric and room-temperature plasma(ARTP)mutagenesis,and the corresponding fermentation process was subsequently optimized.Based on our single-factor experiments and Box-Behnken design results,the optimal fermentation process was conducted with glucose supplementation at 49.4 g/L,peptone supplementation at 21.1 g/L,and initial pH 7.12,leading to cytosine production of 1.43 g/L.This research work established a robust microbial platform for green biosynthesis of cytosine,and could further provide fermentation strategies to enhance its industrial-scale production.关键词
ARTP/诱变/胞嘧啶/发酵条件优化/响应面法/绿色生物合成Key words
atmospheric and room-temperature plasma(ARTP)/mutagenesis/cytosine/optimization of fermentation conditions/response surface methodology/green biosynthesis分类
化学化工引用本文复制引用
孙萌,张云志,夏海华,于冲,张淑梅,闫更轩,田缘,周璐,王子瑄,吴皓琼,任洪发,潘俊豪,赵易灼,刘春燕..基于常压室温等离子体(ARTP)诱变的胞嘧啶高产菌株筛选及其发酵条件优化[J].生物加工过程,2026,24(2):127-134,8.基金项目
黑龙江省重点研发计划(2022ZX02C10) （2022ZX02C10）
