四川医学2026，Vol.47Issue(4)：372-377,6.DOI:10.16252/j.cnki.issn1004-0501-2026.04.003
基于机器学习与WGCNA综合分析CD5L在慢加急性肝衰竭中的免疫调节作用
The Immunomodulatory Role of CD5L in Slow-on-Chronic Acute Liver Failure Based on Machine Learning and WGCNA:a Comprehensive Analysis
摘要
Abstract
Objective To investigate the immunomodulatory role of CD5L in acute-on-chronic liver failure(ACLF)and explore its mechanism of action.Methods Gene expression data from the GSE139602 dataset(GEO)were analyzed via differen-tial expression analysis,GO/KEGG enrichment,immune infiltration profiling,WGCNA,and SVM-RFE to identify key genes in ACLF.CD5L expression was validated via histopathology and qPCR.Results Total 1,080 differentially expressed genes(DEGs)were identified,enriched in pathways such as ECM-receptor interaction,bile secretion,folate metabolism,and cytochrome P450-me-diated detoxification.Neutrophil infiltration levels were significantly higher in ACLF liver tissues(P＜0.05).CD5L was identified as a hub gene,and its expression was significantly downregulated in ACLF tissues(P＜0.05),negatively correlating with lobular structural damage.Conclusion CD5L may contribute to ACLF progression by disrupting immune homeostasis and hepatic meta-bolic balance.It represents a potential diagnostic biomarker and therapeutic target.关键词
慢加急性肝衰竭/CD5L/免疫浸润/SVM-RFE/WGCNA/机器学习Key words
acute-on-chronic liver failure/CD5L/immune infiltration/SVM-RFE/WGCNA/machine learning分类
医药卫生引用本文复制引用
帕成周,曹俊,赵雄齐,陈刚,余杰琦..基于机器学习与WGCNA综合分析CD5L在慢加急性肝衰竭中的免疫调节作用[J].四川医学,2026,47(4):372-377,6.基金项目
国家临床重点专科建设项目 （）
