陕西中医2026，Vol.47Issue(5)：579-584,6.DOI:10.3969/j.issn.1000-7369.2026.05.001
海桐皮方凝胶贴膏对大鼠膝骨关节炎作用机制
Mechanism of action of holly bark gel patch on rats with knee osteoarthritis
摘要
Abstract
Objective:To explore therapeutic effect of the Haidongpi formula gel plaster on knee osteoarthritis(KOA)in rats and the molecular mechanism of regulating the SOCS3/JAK/STAT signaling pathway.Methods:36 8-week-old SPF-grade SD rats were randomly divided into blank group,sham operation group,KOA control group,and treatment group.KOA rat model was established by the Panicker method.The treatment group was treated with Haidongpi formula gel plaster externally,while the KOA control group was treated with blank plasters.The inter-vention lasted for two courses.The mechanical pain threshold(PWT),thermal radiation pain threshold(PWL),and knee joint diameter(KD)of the rats were evaluated by behavioral tests.The levels of IL-1β,IL-6,and TNF-αin ser-um were detected by ELISA.The morphology of cartilage was observed by HE and safranin O-fast green staining.The expression levels of SOCS3,COX-2,JAK2,and STAT3 genes and related proteins in cartilage tissue were detec-ted by RT-qPCR and Western blot.Results:Behavioral tests showed that after 2 weeks of intervention with the Haid-ongpi formula gel plaster,PWL of the treatment group rats recovered,and there was no statistically significant differ-ence compared with the blank group(P>0.05).The PWT showed continuous upward trend,and the degree of knee joint swelling recovered faster than that of the KOA control group.ELISA indicated that the levels of IL-1β,IL-6,and TNF-αin the serum of the treatment group were lower than those in the KOA control group(P<0.01).Histological staining showed that after intervention with the Haidongpi formula gel plaster,surface of the cartilage became smoot-her,structural integrity improved,arrangement of chondrocytes became regular,and safranin O staining recovered significantly,with less loss of proteoglycans.Molecular biological detection showed that the expression of SOCS3 gene and protein in the treatment group was higher than that in the KOA control group,the expression of COX-2 was significantly decreased,and the ratios of p-JAK2/JAK2 and p-STAT3/STAT3 decreased(P<0.01).Conclusion:Haidongpi formula gel plaster can relieve pain and joint swelling in KOA rats and protect joint cartilage.The mecha-nism may be related to the upregulation of SOCS3 expression,enhancing the negative regulation of the JAK/STAT signaling pathway,and inhibiting the release of downstream pro-inflammatory factors.关键词
海桐皮方凝胶贴膏/膝骨关节炎/细胞因子信号转导抑制因子 3/Janus 激酶/信号转导和转录激活蛋白Key words
Haitongpi formula gel patch/Knee osteoarthritis/SOCS3/JAK/STAT分类
医药卫生引用本文复制引用
夏迪,张清,李法杰,侯晓宙,杨满红,万田豪,谷金玉..海桐皮方凝胶贴膏对大鼠膝骨关节炎作用机制[J].陕西中医,2026,47(5):579-584,6.基金项目
国家自然科学基金青年基金资助项目(82505627) （82505627）
北京市自然科学基金资助项目(7244503) （7244503）
首都卫生发展科研专项项目(首发2024-1-7161) （首发2024-1-7161）
