陕西中医2026，Vol.47Issue(5)：591-597,7.DOI:10.3969/j.issn.1000-7369.2026.05.003
丹蛭降糖胶囊调控ASK1-JNK信号通路对糖尿病大鼠主动脉粥样硬化干预机制
Intervention mechanism of Danzhi Jiangtang capsule in regulating ASK1-JNK signaling pathway for atherosclerosis in aorta of diabetic rats
摘要
Abstract
Objective:To explore effect of Danzhi Jiangtang capsules on apoptosis signal-regulating kinase 1-c-Jun N-terminal kinase(ASK1-JNK)signaling pathway in SD rats with diabetes mellitus(DM)combined with ather-osclerosis(AS).Methods:SD rats were used to establish DM combined with AS model with streptozotocin(STZ)so-lution and N-nitro-L-arginine methyl ester(L-NAME).The successfully modeled rats were randomly divided into the model group,atorvastatin group,and Danzhi Jiangtang capsules high-dose group 1.08 g/(kg·d),medium-dose group 0.54 g/(kg·d),and low-dose group 0.27 g/(kg·d),with a blank group also set up.Each drug administration group was given corresponding drug by gavage,while the model group and blank group were given 0.9%NaCl solu-tion of the same volume.After 8 weeks of intervention,the relevant indicators were detected.Results:Compared with the blank group,the model group had significantly increased fasting plasma glucose(FPG),triglycerides(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),and endothelin-1(ET-1)(all P<0.01),and significantly decreased high-density lipoprotein cholesterol(HDL-C)and nitric oxide(NO)(all P<0.01).The relative expression of ASK1,p-ASK1,JNK,p-JNK,and c-Jun proteins in the aorta was significantly upregulated(all P<0.01).Compared with the model group,Danzhi Jiangtang capsules significantly reduced FPG,decreased TG,TC,and LDL-C,increased HDL-C,inhibited TNF-αand IL-6 lev-els,increased NO and decreased ET-1 content,with statistically significant differences(all P<0.01).HE staining showed that the high-and medium-dose groups of Danzhi Jiangtang capsules significantly alleviated aortic endothelial rupture,smooth muscle disorder,and intimal thickening.Western blot confirmed that Danzhi Jiangtang capsules in-hibited the expression of key proteins in the ASK1-JNK pathway(ASK1,p-ASK1,JNK,p-JNK,and c-Jun),with sta-tistically significant differences(all P<0.01).Conclusion:Danzhi Jiangtang capsules improve glucose and lipid me-tabolism,reduce inflammatory response,alleviate vascular endothelial injury,and delay the progression of atheroscle-rosis in rats by inhibiting the expression of the ASK1-JNK signaling pathway.关键词
丹蛭降糖胶囊/糖脂代谢/c-Jun 氨基末端激酶/凋亡信号调节激酶 1/动脉粥样硬化/糖尿病并发症Key words
Danzhi Jiangtang capsule/Glucose and lipid metabolism/c-jun N-terminal protein kinases/Apop-tosis signal-regulating kinase 1/Atherosclerosis/Diabetic complications分类
医药卫生引用本文复制引用
巫玉童,张栋飞,金磊,张智新,彭静静,徐甜,方朝晖..丹蛭降糖胶囊调控ASK1-JNK信号通路对糖尿病大鼠主动脉粥样硬化干预机制[J].陕西中医,2026,47(5):591-597,7.基金项目
国家自然科学基金资助项目(82174153,81774286,81573944) （82174153,81774286,81573944）
安徽省卫生健康科研项目(AHWJ2023BAc20123) （AHWJ2023BAc20123）
合肥综合性国家科学中心大健康研究院"揭榜挂帅"项目(2023CXMMTCM003) （2023CXMMTCM003）
