陕西中医2026，Vol.47Issue(5)：598-602,613,6.DOI:10.3969/j.issn.1000-7369.2026.05.004
电针调控脊髓背角α2δ-1/NR2B和ERK5蛋白表达改善大鼠神经病理性疼痛作用机制
Mechanism of electroacupuncture regulating expression of α2δ-1/NR2B and ERK5 proteins in spinal dorsal horn to improve neuropathic pain in rats
摘要
Abstract
Objective:To explore mechanism by which electroacupuncture regulates expression of voltage-gated calcium channelα2δ-1 subunit(α2δ-1),N-methyl-D-aspartate receptor 2B subtype(NR2B),and extracellular signal-regulated kinase 5(ERK5)in the spinal dorsal horn to alleviate neuropathic pain(NP)and associated depressive be-haviors in rats.Methods:Forty male SD rats were randomly divided into four groups:blank group,model group,the sham electroacupuncture group,and the electroacupuncture group,with 10 rats in each group.Except for the blank group,the other groups were subjected to chronic constriction injury(CCI)of the sciatic nerve.On the 7th day after modeling,the electroacupuncture group received electroacupuncture at the right Huantiao and Yanglingquan points,while the sham electroacupuncture group received superficial subcutaneous needling at non-acupoints without electri-cal stimulation,for 30 minutes each time,for 7 consecutive days.The mechanical paw withdrawal threshold(PWMT),thermal paw withdrawal latency(PWTL),sucrose preference rate,total distance traveled in the open field test,and the number of grid crossings were observed in each group.Western blot and RT-qPCR techniques were used to detect protein and mRNA expression levels ofα2δ-1,NR2B,and ERK5 in the spinal dorsal horn.Results:Compared with the blank group,the PWMT and PWTL values,sucrose preference rate,total distance traveled in the open field test,and the number of grid crossings in the model group decreased(all P<0.05),and the protein and mRNA ex-pression levels of α2δ-1,NR2B,and ERK5 in the spinal dorsal horn increased(all P<0.05).Compared with the model group,the PWMT and PWTL values,sucrose preference rate,total distance traveled in the open field test,and the number of grid crossings in the electroacupuncture group increased,and the protein and mRNA expression levels ofα2δ-1,NR2B,and ERK5 in the spinal dorsal horn decreased(all P<0.05).Compared with the model group,the above changes in the sham electroacupuncture group were not significant(P>0.05).Conclusion:Electroacupuncture can effectively alleviate hyperalgesia and improve depressive behaviors in CCI model rats,and its mechanism may be related to the down-regulation of abnormal expression of α2δ-1 and NR2B in the spinal dorsal horn and the interven-tion of the ERK5 pathway.关键词
神经病理性疼痛/电针/脊髓背角/N-甲基-D-天冬氨酸 2B 受体/细胞外信号调节激酶 5/抑郁Key words
Neuropathic pain/Electroacupuncture/Dorsal horn of the spinal cord/N-methyl-D-aspartate re-ceptor subtype 2B/Extracellular signal-regulated kinase 5/Depression分类
医药卫生引用本文复制引用
乐薇,辛欢,胡欢,樊展,吴贻森,黄玉兰,杨格格..电针调控脊髓背角α2δ-1/NR2B和ERK5蛋白表达改善大鼠神经病理性疼痛作用机制[J].陕西中医,2026,47(5):598-602,613,6.基金项目
湖北省中医药管理局中医药科研项目(ZY2025L024) （ZY2025L024）
湖北省自然科学基金联合基金资助项目(2025AFD608) （2025AFD608）
