时珍国医国药2026，Vol.37Issue(8)：1401-1413,13.DOI:10.70976/j.1008-0805.SZGYGY-2026-0801
杜仲-牛膝配伍抗类风湿关节炎机制的网络药理学和实验验证研究
Metwork pharmacology and experimental validation of the anti-rheumatoid arthritis mecha-nism of Cortex Eucommiae-Radix Achyranthis Bidentatae medicinal couplet
摘要
Abstract
Objective To explore the mechanism of the Cortex Eucommiae(CE)-Radix Achyranthis Bidentatae(RAB)medicinal cou-plet against rheumatoid arthritis(RA).Methods A collagen-induced arthritis(CIA)rat model was established.Paw swelling volume and arthritis scores were recorded after drug administration.HE staining and safranin O/fast green staining were used to observe ankle joint pathology.Serum levels of bone metabolism markers and inflammatory cytokines were detected.Scratch wound healing and Tran-swell assays were conducted to evaluate the migration and invasion capabilities of HFLS-RA cells.Tartrate-resistant acid phosphatase(TRAP)staining was employed to assess osteoclast differentiation of RAW264.7 cells.The mRNA levels of related factors were deter-mined by reverse transcription-quantitative polymerase chain reaction(RT-qPCR).Results Both individual herbs(CE and RAB)and their medicinal couplet(CE-RAB)reduced the paw swelling volume and arthritis scores in CIA rats.CE and CE-RAB reduced serum levels of β-cross-linked C-telopeptide of type I collagen(β-CTX),pyridinoline cross-linked carboxy-terminal telopeptide of type I col-lagen(ICTP),nitric oxide(NO),IL-1β,and TNF-α,while increasing the levels of procollagen type I N-terminal propeptide(PINP)and bone gla protein(BGP).In spleen tissue,CE,RAB,and CE-RAB downregulated the mRNA expression of IL-6,IL-1β,and TNF-α.Additionally,RAB and CE-RAB upregulated the mRNA expression of IL-10.CE,RAB,and CE-RAB inhibited the migration of HFLS-RA cells and downregulated the mRNA expression of IL-6,IL-1β,and TNF-α in these cells.Furthermore,CE and CE-RAB also inhibited cell invasion.CE,RAB,and CE-RAB suppressed the differentiation of RAW264.7 cells into osteoclasts.Moreover,CE and CE-RAB reduced the mRNA expression of IL-6,IL-1β,and TNF-α in RAW264.7 cells.CE,RAB,and CE-RAB downregulated IL-17A mRNA levels in the ankle joints of CIA rats and in HFLS-RA cells.Conclusion The CE-RAB alleviated arthritis in CIA rats,mitigated bone metabolism disorder and pathological damage,suppressed the migration,invasion,and inflammatory factor expression in HFLS-RA cells,and inhibited osteoclast differentiation.The synergistic mechanism likely involves the TNF and IL-17 signaling path-ways,with CE appearing to play a predominant role.关键词
杜仲/牛膝/中药配伍/类风湿关节炎Key words
Cortex Eucommiae/Radix Achyranthis Bidentatae/Medicinal couplet/Rheumatoid arthritis分类
医药卫生引用本文复制引用
杨若妍,陈迎愿,吴梦婷,王健英,张磊,王凯,袁颖..杜仲-牛膝配伍抗类风湿关节炎机制的网络药理学和实验验证研究[J].时珍国医国药,2026,37(8):1401-1413,13.基金项目
国家自然科学基金面上项目(82374052) （82374052）
