时珍国医国药2026，Vol.37Issue(8)：1431-1438,8.DOI:10.70976/j.1008-0805.SZGYGY-2026-0804
疏肝止泻方经VIPR2/PLC/PKC通路改善肝郁脾虚型IBS-D大鼠肠道通透性的机制研究
Mechanism of Shugan Zhixie Formula(疏肝止泻方)in improving intestinal permeability in IBS-D rats with liver depression and spleen deficiency syndrome via the VIPR2/PLC/PKC pathway
摘要
Abstract
Objective To examine,using intestinal permeability and the VIPR2/PLC/PKC signaling pathway,the intervention impact and mechanism of the liver-soothing and spleen-strengthening technique on diarrhea-predominant irritable bowel syndrome(IBS-D)with liver depression and spleen deficiency in rat models.In order to create a rat model that better satisfies the needs of integrating normal IBS-D symptoms with emotional stress disorders,it is also necessary to refine the modeling approach for IBS-D rats with liver depression and spleen deficiency.Methods Seven of the fifty SPF-grade Wistar rats were placed in the blank group.The remaining rats were split into the model group,pinaverium bromide group,and low-,medium-,and high-dose Shugan Zhixie Formula(疏肝止泻方,SGZXF)groups following successful model replication.Blood and colon tissues were obtained for associated indicator testing and analy-sis following a 14-day continuous intervention.Results According to ELISA,the other groups'plasma D-lactate,serum PIP-2,and DAG levels increased to differing degrees when compared to the blank group(P<0.001).PI3K protein and mRNA expression declined(P<0.001),whereas VIPR2,PLC,and PKC protein and mRNA expression levels were dramatically increased(P<0.001).Zo-1 and occludin mRNA and protein expression were dramatically decreased(P<0.01).Furthermore,the protein and mRNA expression levels of the low-,medium-,and high-dose SGZXF groups differed significantly from the model group(P<0.05).Lastly,immunohis-tochemistry(IHC)revealed that each group's hypothalamus had higher average fluorescence intensity of VIP and VIPR2 proteins than the blank group(P<0.01,P<0.05).Furthermore,the rat model was effectively constructed using the optimal modeling approach.Conclusion Intestinal acetic acid perfusion,erratic feeding,and restraint-tail clamping stress can be used to create a rat model of IBS-D with liver depression and spleen deficiency.By controlling the expression of proteins in the VIPR2/PLC/PKC signaling pathway,the Liver-soothing and Spleen-strengthening approach improves intestinal permeability.关键词
腹泻型肠易激综合征/肝郁脾虚证/VIPR2/PLC/PKC信号通路/疏肝健脾法/肠道通透性/疏肝止泻方Key words
Diarrhea-predominant irritable bowel syndrome(IBS-D)/Liver depression and spleen deficiency syndrome/VIPR2/PLC/PKC signaling pathway/Liver-soothing and spleen-fortifying method/Intestinal permeability/Shugan Zhixie Formula(疏肝止泻方)分类
医药卫生引用本文复制引用
刘俊宏,李明,张娟,王淼蕾,李亚静,付兆媛,毛兰芳,郭欣..疏肝止泻方经VIPR2/PLC/PKC通路改善肝郁脾虚型IBS-D大鼠肠道通透性的机制研究[J].时珍国医国药,2026,37(8):1431-1438,8.基金项目
国家中医药管理局青年岐黄学者基金项目(国中医药人教函[2022]256号) （国中医药人教函[2022]256号）
甘肃省临床医学研究中心建设项目(21JR7RA682) （21JR7RA682）
甘肃省科技厅联合科研项目基金(24JRRA874) （24JRRA874）
甘肃省科技厅重点研发计划(社会发展类)(22YF7FA100) （社会发展类）
甘肃省卫生健康行业科研计划项目(GSWSQN2022-01) （GSWSQN2022-01）
