时珍国医国药2026，Vol.37Issue(8)：1448-1455,8.DOI:10.70976/j.1008-0805.SZGYGY-2026-0806
精天颗粒拆方调控Wnt/β-catenin通路改善小鼠慢性肾纤维化的作用及其机制研究
Role and mechanism of Jingtian Granule(精天颗粒)disassembled formula in ameliorating-chronic renal fibrosis in mice by modulating the Wnt/β-catenin pathway
摘要
Abstract
Objective To study the effect of Jingtian Granule(精天颗粒,JTG)disassembled formula on chronic renal fibrosis in mice and its mechanism of action.Methods C57BL/6 mice were randomly divided into the blank group,model group,Dahuang Gancao Decoction(大黄甘草汤,DHGCD)group,Wuling Powder(五苓散,WLP)group,Huangjing Hongjingtian Chuangshanlong(黄精红景天穿山龙,HHC)group,JTG group and positive drug group,with 8 mice in each group.In addition to the blank group,each group was fed with 0.2%adenine feed for 4 weeks to establish the chronic renal fibrosis model,and from the 3rd week onwards,each group was treated with the corresponding drugs for 2 weeks in addition to the blank group and the model group.General signs of mice were observed;creatinine and urea levels in serum were detected;pathological changes in renal tissues were detected by hematoxylin-eosin(HE)and periodate-schiff(PAS)staining;pathological changes in renal fibers were observed by Masson trichrome staining;and Wnt3 in renal tissues of mice in each group was detected by Western blot(WB).WB was used to detect the expression levels of Wnt3a and β-catenin,α-smooth muscle actin(α-SMA),collagen type I(ColⅠ)and collagen type Ⅲ(ColⅢ)in the kidney tissues of mice.Results JTG and its disassembled formula significantly improved the signs of mice with renal fibrosis,reduced the levels of urea and cre-atinine in the serum of mice with chronic renal fibrosis(P<0.01,P<0.01),improved the histopathological changes of kidneys,and down-regulated the protein expression of Wnt3a,β-catenin,ColⅠ,ColⅢ,α-SMA(P<0.05).The comprehensive efficacy of the whole formula group was optimal,and the disassembled formula group had its own advantages in different indexes.Conclusion JTG dis-assembled formula can effectively inhibit the excessive deposition of extracellular matrix(ECM)and alleviate chronic renal fibrosis through the regulation of Wnt/β-catenin pathway.关键词
精天颗粒/慢性肾纤维化/细胞外基质沉积/Wnt/β-Catenin信号通路Key words
Jingtian Granule(精天颗粒)/Chronic renal fibrosis/Extracellular matrix deposition/Wnt/β-Catenin signaling pathway分类
医药卫生引用本文复制引用
李成银,惠淼鑫,巴元明..精天颗粒拆方调控Wnt/β-catenin通路改善小鼠慢性肾纤维化的作用及其机制研究[J].时珍国医国药,2026,37(8):1448-1455,8.基金项目
国家中医药管理局第六批全国中医临床优秀人才研修项目(国中医药人教函[2025]256号) （国中医药人教函[2025]256号）
国家中医药管理局全国名老中医药专家传承工作室建设项目(国中医药人教函[2022]75号) （国中医药人教函[2022]75号）
湖北省自然科学基金重点项目(2022CFD023) （2022CFD023）
湖北中医药大学2025年校级研究生工作站建设点项目[华沃生物科技(武汉)有限公司工作站,中医研[2025]50号]. （武汉）
