时珍国医国药2026，Vol.37Issue(8)：1456-1467,12.DOI:10.70976/j.1008-0805.SZGYGY-2026-0807
基于UPLC-Q-TOF-MS/MS的安体威颗粒化学成分及入血成分分析
Analysis of chemical constituents and blood-absorbed constituents of Antiwei Granule(安体威颗粒)based on UPLC-Q-TOF-MS/MS
摘要
Abstract
Objective To establish a method based on ultra-high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MS/MS)for analyzing the chemical constituents and blood-absorbed constituents of Antiwei Granule(安体威颗粒,ATWG).Methods The analysis was performed on an ACQUITY UPLC HSS T3 column(2.1 mm×100 mm,1.7 μm)maintained at 30℃.The mobile phase consisted of 0.1%formic acid in water(A)and acetonitrile(B)with a flow rate of 0.3 mL/min in a gradient elution mode.An electrospray ionization(ESI)source was used for detection in both positive and negative ion scanning modes.Constituents were identified based on their exact molecular ions,retention times,and MS/MS fragment ions,with reference to standard substance information and a self-built database.Results A total of 55 chemical constituents were identified from the granules in vitro.From rat serum samples,37 constituents were detected,of which 22 were prototype constituents and 15 were metabolites.Conclusion A rapid and reliable analytical method was established for identifying the chemical constituents of ATWG both in vitro and in vitro.This method lays a foundation for clarifying the pharmacodynamic material basis and improving the quality stan-dards of ATWG.关键词
安体威颗粒/化学成分/入血成分/超高效液相色谱-四极杆-飞行时间串联质谱法Key words
Antiwei Granule(安体威颗粒)/Chemical constituents/Blood-absorbed constituents/Ultra-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry分类
医药卫生引用本文复制引用
白其慧,李云鹃,马文涛,何毅,周桂荣,缪兴龙..基于UPLC-Q-TOF-MS/MS的安体威颗粒化学成分及入血成分分析[J].时珍国医国药,2026,37(8):1456-1467,12.基金项目
天津市科技计划项目(24ZYJDSS00210) （24ZYJDSS00210）
