时珍国医国药2026，Vol.37Issue(8)：1476-1482,7.DOI:10.70976/j.1008-0805.SZGYGY-2026-0809
高抗逆性纤维素酶产生菌降解复方药渣研究
The degradation of compound medicinal residue by high stress resistance strains
摘要
Abstract
Objective Screening strains with high stress resistance cellulase-production for improve the resource utilization rate of com-pound medicinal residues.Methods High-yield cellulase strains were screened from the rotten compound medicine residues,and were identified through ITS sequence analysis.The fermentation process was optimized through single factors and response surfaces.The per-formance of the strain in degrading compound medicine residues was investigated.Results A fungal strain CKT2 with high cellulose-producing and tolerance to the residue of compound medicine residues was screened,and ITS sequence analysis identified as Penicillium sp.The stress resistance results showed that CKT2 could grow in pH 5.0,250 g/L PEG6000,and 60 μg/mL extracts.Through single-factor and response surface optimization,the optimal cellulase production parameters were:the addition residues 2.8%,inoculation amount 1.43%,initial pH 4.60,rotational speed 150 r/min.Under this optimal condition,the filter paper enzyme,endoglucanase and xylanase activities of Penicillium sp.CKT2 were 13.18 U/mL,107.91 U/mL and 217.91 U/mL,respectively.The degradation rate of Penicillium sp.CKT2 enzymatic hydrolysis compound medicine residues was 45.87%.Conclusion Penicillium sp.CKT2 has good envi-ronmental adaptability and high yield of cellulase,and has a good degradation effect on compound medicine residues.关键词
复方药渣/高抗逆性/响应面/纤维素酶/木质纤维素降解Key words
Compound medicinal residue/High stress resistance/Response surface/Cellulase/Degradation of lignocellulose分类
资源环境引用本文复制引用
权静,常思源,恽辰珂,徐天韵,陈天翼,张媛,杨秉乾,何志豪,李冰峰,张森..高抗逆性纤维素酶产生菌降解复方药渣研究[J].时珍国医国药,2026,37(8):1476-1482,7.基金项目
国家自然科学基金(82574719) （82574719）
江苏高校"青蓝工程"(2022年) （2022年）
国家中医药管理局创新团队与人才培养计划(ZYYCXTD-D-202005) （ZYYCXTD-D-202005）
中华中医药学会青年人才托举工程(2022-QNRC2-B18) （2022-QNRC2-B18）
