上海海洋大学学报2026，Vol.35Issue(3)：614-624,11.DOI:10.12024/jsou.20250204781
vasa基因在三角帆蚌性腺发育中的表达
Expression of vasa gene in Hyriopsis cumingii during gonadal development
摘要
Abstract
To investigate the role of the vasa gene in gonadal development of the triangle sail mussel(Hyriopsis cumingii),techniques including quantitative real-time PCR(qRT-PCR)and in situ hybridization were employed to analyze the structural characteristics and spatiotemporal expression patterns of the vasa gene in male and female tissues,as well as during early and mature developmental stages of testes and ovaries.The results revealed that the vasa gene cDNA consisted of 4 673 bp,encoding 808 amino acids,containing two functional domains(DEXDc and HELICc)and nine conserved motifs characteristic of the DEAD-box protein family.The vasa gene exhibited significantly higher expression in ovaries and testes compared to other tissues(P<0.05).During early gonadal development,its expression showed an initial increase followed by a decline,reaching significant elevation at 6 months of age with marked differences compared to other age groups(P<0.05).In mid-to-late gonadal development,vasa expression displayed a progressive upward trend,with significantly higher levels in females than in males(P<0.01).In situ hybridization localized vasa expression to oocytes,spermatogonia,spermatocytes,sperm cells,follicle walls,as well as primordial germ cells and follicular cells in juvenile gonads.This study suggests that the vasa gene participates in gonadal development in H.cumingii,potentially regulating primordial germ cell proliferation and gamete meiosis,serving as a putative regulatory factor in gametogenesis,and playing crucial roles in maintaining testicular/ovarian and germ cell development.These findings provide theoretical insights for research on gonadal development,gametogenesis,and monosex breeding techniques in H.cumingii.关键词
三角帆蚌/vasa基因/性腺发育/配子发生Key words
Hyriopsis cumingii/vasa gene/gonadal development/gametogenesis分类
农业科技引用本文复制引用
任怡锦,汪建伟,毛颖睿,汪桂玲..vasa基因在三角帆蚌性腺发育中的表达[J].上海海洋大学学报,2026,35(3):614-624,11.基金项目
国家自然科学基金(32072975,32573497) （32072975,32573497）
国家重点研发计划(2022YFD2400700) （2022YFD2400700）
