畜牧兽医学报2026，Vol.57Issue(4)：2048-2059,12.DOI:10.11843/j.issn.0366-6964.2026.04.023
肉桂醛对小鼠卵泡颗粒细胞雌激素分泌及其凋亡的影响
The Effect of Cinnamaldehyde on Estrogen Secretion and Apoptosis of Mouse Ovarian Granulosa Cells
摘要
Abstract
Ovarian granulosa cells(GCs)of mice are the core cells responsible for estrogen secretion.This study aimed to investigate the effect of cinnamaldehyde treatment on estrogen secretion and apoptosis of mouse ovarian GCs.In this study,3-week-old SPF-grade female KM mice were selected.The mouse ovarian GCs were isolated and divided into a blank control group,a 5× 10-5 mol·L-1 cinnamaldehyde treatment group,and a 10×10-5 mol·L-1 cinnamaldehyde treatment group.Each group had 3 replicates.Firstly,this study analyzed the gene expression profiles of cinnamaldehyde-treated mouse follicular GCs and the enrichment of differentially expressed genes(DEGs)using transcriptome sequencing analyses.Then,ELISA,cell counting Kit-8(CCK-8),flow cytometry,and RT-qPCR were employed to clarify the effect of cinnamaldehyde on estrogen secretion and apoptosis of mouse GCs.The sequencing results showed that the DEGs in mouse ovarian GCs treated with 5×10-5 mol·L-1 cinnamaldehyde were enriched in the PI3K-Akt signaling pathway,tumor-related pathways,AGE-RAGE signaling pathway in diabetic complications,and progesterone-mediated oocyte maturation,among others.The experimental results showed that treatment of mouse ovarian granulosa cells GCs with 5×10-5 mol·L-1 cinnamaldehyde could promote estrogen secretion by significantly upregulating the relative mRNA expression levels of StAR,CYP19A1,and CYP11A1.Compared with the control group,treatment with 5×10-5 mol·L-1 and 10×10-5 mol·L-1 cinnamaldehyde both induced apoptosis of mouse ovarian GCs to different degrees.The 5×10-5 mol·L-1 cinnamaldehyde treatment significantly upregulated the relative mRNA expression levels of CHOP,IRE1,ATF6,and PERK,while 10×10-5 mol·L-1 cinnamaldehyde treatment significantly upregulated the relative mRNA expression levels of CHOP and ATF6.In conclusion,5×10-5 mol·L-1 cinnamaldehyde may promote estrogen secretion in mouse ovarian GCs by upregulating the expression of steroid hormone synthases via the PI3K-Akt signaling pathway.Additionally,both 5×10-5 mol·L-1 and 10×10-5 mol·L-1 cinnamaldehyde can induce GCs apoptosis through the endoplasmic reticulum stress(ERS)pathway.关键词
肉桂醛/小鼠卵泡颗粒细胞/雌激素/类固醇激素合成酶/内质网应激Key words
cinnamaldehyde/mouse ovarian granulosa cells/estrogen/steroid hormone synthases/endoplasmic reticulum stress分类
农业科技引用本文复制引用
郭雨欣,林双秀,傅昌盛,杨洋,王登峰,郭盼盼,吴海燕,殷光文,王磊..肉桂醛对小鼠卵泡颗粒细胞雌激素分泌及其凋亡的影响[J].畜牧兽医学报,2026,57(4):2048-2059,12.基金项目
国家自然科学基金(32102734) （32102734）
福建省中青年教师教育科研项目(JAT210659) （JAT210659）
福建农林大学校科技创新专项基金项目(KFB25008A) （KFB25008A）
