西北农林科技大学学报（自然科学版）2026，Vol.54Issue(5)：66-78,120,14.DOI:10.13207/j.jnwafu.2026.05.007
基于多组学分析水生蔬菜豆瓣菜对镉胁迫逆境的响应
Multi-omics analysis of watercress response to cadmium stress
摘要
Abstract
[Objective]The study aims to explore the mechanisms underlying watercress(Nasturtium offi-cinale)response to Cadmium(Cd)stress,to provide a reference basis for mitigating Cd toxicity and advancing research on plant resistance to heavy metals.[Method]Hydroponically grown watercress seedlings were treated with 0,0.2,0.8,3.2,and 12.8 mg/L CdCl2 to determine the appropriate Cd concentration.The effects of Cd stress on growth,physiological indices,metabolite levels,and gene expression were investigated.[Result]①Under 0.8 mg/L CdCl2 stress,biomass and photosynthesis of watercress were significantly inhibited.Sulfur(S)content was decreased,while Cd,Fe,Mn,K,and Ca contents were increased.Activities of POD and SOD were declined,whereas MDA levels were increased.Additionally,root vascular bundle area was decreased,cell walls were thickened,and intracellular structures,such as plastids and mitochondria,were damaged.② Cd stress downregulated the expression of the genes associated with the MAPK signaling pathway(133 genes)and phen-ylpropanoid biosynthesis pathway(104 genes),impairing stress responses and cell wall reinforcement.It also suppressed the expression of genes related to glycolysis and gluconeogenesis,affecting energy metabolism,while simultaneously upregulating the expression of genes involved in peroxisome activity,alleviating oxidative dam-age.③ Cd stress inhibited the synthesis of metabolites related to glycolysis,starch,and sucrose metabolism.[Conclusion]0.8 mg/L CdCl2 stress significantly inhibited the growth and photosynthesis of watercress.Tran-scriptomic and metabolomic analyses showed that Cd affected the accumulation of carbon metabolites by inhibit-ing the activity of the antioxidant system and the expression of genes related to the carbon metabolism pathway,ultimately hindering the growth of watercress.关键词
豆瓣菜/镉胁迫/糖酵解/糖异生途径/过氧化物酶体途径Key words
watercress/Cd stress/glycolysis/gluconeogenesis/peroxisome分类
农业科技引用本文复制引用
赖紫欣,谭皖湘,骆善伟,李桂花,徐振江,江院..基于多组学分析水生蔬菜豆瓣菜对镉胁迫逆境的响应[J].西北农林科技大学学报（自然科学版）,2026,54(5):66-78,120,14.基金项目
农业农村部申请保护品种DUS测试及已知品种库维护项目(h20230603) （h20230603）
广东省农业科学院创新基金-产业专项"纳米二硫化钼降低芥菜镉吸附机制研究"(202230) （202230）
