西南农业学报2026，Vol.39Issue(2)：235-243,9.DOI:10.16213/j.cnki.scjas.2026.2.002
枯草芽孢杆菌XH-1对稻瘟病菌抑菌活性分析及田间防效初探
Antifungal activity of Bacillus subtilis XH-1 against Magnaporthe oryzae and preliminary field efficacy evaluation
摘要
Abstract
[Objective]The present paper aimed to explore the application potential of Bacillus subtilis strain XH-1 in the biocontrol of rice blast.[Method]The antifungal activity of XH-1 against Magnaporthe oryzae was assessed using a plate confrontation assay.The effects of XH-1 on various stages of the fungus,including conidial production(evaluated on complete medium,CM),conidial germination(induced on hydro-phobic slides),development of infection structures(induced by leaf sheath inoculation),and pathogenicity(via spray inoculation)were in-vestigated.The application potential of XH-1 was further analyzed through field efficacy trials.[Result]The plate confrontation analysis be-tween XH-1 and Guy11 showed that XH-1 could inhibit the colony growth of Guy11,with an inhibition rate of 85.75％.When XH-1 was added to CM medium,the colony growth of M.oryzae was severely inhibited,with inhibition rates of 99.55％(1×),90.91％(10×)and 50.23％(100 ×),respectively,while conidiation was sharply curtailed.Spore-germination assays revealed that XH-1 not only blocks conidi-al germination but also suppresses appressorium formation.Observation of sheath infection showed that XH-1 fermentation broth could effec-tively inhibit the infection process of Guy11.Pathogenicity analysis showed that XH-1 fermentation broth could inhibit the pathogenicity of Guy11 on rice.The field control effect test showed that XH-1 fermentation broth can suppress the onset of rice blast in the field.[Conclu-sion]The B.subtilis XH-1 demonstrates promising potential against rice blast and shows considerable application prospects,providing a valua-ble resource for the biological control of this disease.关键词
稻瘟病/生物防治/枯草芽孢杆菌/田间防效Key words
Rice blast/Biological control/Bacillus subtilis/Field control efficacy分类
农业科技引用本文复制引用
黄昱轲,钟玲,杨永浩,高馨,姚威言,宋洁潞,景帆,许有嫔..枯草芽孢杆菌XH-1对稻瘟病菌抑菌活性分析及田间防效初探[J].西南农业学报,2026,39(2):235-243,9.基金项目
攀西特色作物研究与利用四川省重点实验室(SZKF202408) （SZKF202408）
西南野生动植物资源保护教育部重点实验室(XNYB23-02) （XNYB23-02）
四川省科技厅自然基金项目(2024NSFSC1312) （2024NSFSC1312）
西华师范大学博士启动基金(21E042) （21E042）
西华师范大学自然基金项目(23kc009) （23kc009）
