西南农业学报2026，Vol.39Issue(2)：244-256,13.DOI:10.16213/j.cnki.scjas.2026.2.003
Bacillus velezensis-1功能验证及西瓜枯萎病防治转录组分析
Functional verification of Bacillus velezensis-1 and transcriptome analysis for Fusarium wilt of watermelon control
摘要
Abstract
[Objective]The study aimed to obtain a biocontrol strain that enhanced watermelon resistance to Fusarium wilt of watermelon,and to investigate the antifungal substances produced by the strain and its impact on disease resistance responses in watermelon after inoculation.[Method]Dominant strains were isolated and screened from the rhizosphere of healthy plants.The strain was identified,and its growth condi-tions were determined.Antifungal effects were observed microscopically,and the inhibition mechanisms of Bacillus velezensis-1(hereinafter referred to as BVS-1)were explored through growth-promotion tests,detection of antifungal substance secretion,and transcriptome analysis of watermelon after inoculation with the strain.[Result]BVS-1 was identified as B.velezensis.Its optimal growth conditions were pH 7,30 ℃,beef extract powder as the nitrogen source,yeast extract as the carbon source,and a salt concentration of 10 g/L.The plate confrontation assay showed an inhibition rate of 60％against Fusarium oxysporum f.sp.niveum(Fon),effectively suppressing mycelial growth.Scanning elec-tron microscopy revealed that BVS-1 caused hyphal rupture,distortion and deformation.Extracellular enzyme tests indicated that BVS-1 secre-ted protease,amylase and cellulase.It could synthesize auxin,solubilize potassium,fix nitrogen and produce siderophores.Antimicrobial vola-tile organic compounds such as 2,4-di-tert-butylphenol,stearic acid and pentadecanoic acid were detected.After inoculation,significant differences were observed in watermelon metabolic pathways,including phenylalanine metabolism,α-linolenic acid metabolism,and glutathi-one metabolism,with upregulation of the photosynthesis-antenna protein pathway.[Conclusion]B.velezensis BVS-1 demonstrates strong anti-fungal activity against Fusarium oxysporum,significantly enhances watermelon resistance to Fusarium wilt,and shows promising application potential.This strain can serve as a valuable resource for the biological control of watermelon Fusarium wilt.关键词
西瓜枯萎病/贝莱斯芽孢杆菌/胞外酶/抑菌性有机挥发物/转录组Key words
Fusarium wilt of watermelon/Bacillus velezensis/Extracellular enzymes/Antimicrobial volatile organic compounds/Transcriptome分类
农业科技引用本文复制引用
钱文浩,郭东升,周鹏飞,陈丽萍..Bacillus velezensis-1功能验证及西瓜枯萎病防治转录组分析[J].西南农业学报,2026,39(2):244-256,13.基金项目
浙江省蔬菜产业技术团队项目(浙农科发[2023]13号) （浙农科发[2023]13号）
湖州市科技项目(3305004022141) （3305004022141）
