中草药2026，Vol.57Issue(8)：3121-3141,21.DOI:10.7501/j.issn.0253-2670.2026.08.024
基于代谢组和转录组的酸枣仁活性成分生物合成关键基因的初步筛选研究
Preliminary screening of key genes for biosynthesis of active components of Ziziphi Spinosae Semen based on metabolome and transcriptome
摘要
Abstract
Objective This study aimed to comprehensively identify the key genes involved in the biosynthesis of spinosin and jujuboside A.Methods Ziziphi Spinosae Semen samples at green fruit and mature stages were selected as experimental materials to construct transcriptomic and metabolomic datasets.Key candidate genes associated with the spinosin and jujuboside A biosynthesis pathways were systematically analyzed through functional annotation and differential expression gene analysis.Results Metabolomic profiling detected 1 646 metabolites,while transcriptomic sequencing and functional annotation identified 7 316 differentially expressed genes.Results revealed that spinosin was relatively enriched during the green fruit stage of Ziziphi Spinosae Semen,whereas jujuboside A accumulated predominantly at the mature stage.Through phylogenetic and heatmap analyses,50 key candidate genes were screened,including 33 UGTs,12 OMTs,two OSCs,and three CYP450s,which are hypothesized to participate in the downstream biosynthesis of spinosin and jujuboside A.Conclusion This work systematically deciphers and proposes biosynthetic pathways for these components,laying a foundation for subsequent functional gene validation.It advances molecular biology research on Ziziphi Spinosae Semen bioactive compounds and provides novel insights for the development and utilization of traditional Chinese medicine resources.关键词
酸枣仁/代谢组学/转录组学/斯皮诺素/酸枣仁皂苷AKey words
Ziziphus jujube Mill.var.spinosa(Bunge)Hu ex H.F.Chou/metabolomics/transcriptomics/spinosin/jujuboside A分类
医药卫生引用本文复制引用
陈继聪,孙航,练从龙,马蕊,赵乐,陈随清,刘秀玉..基于代谢组和转录组的酸枣仁活性成分生物合成关键基因的初步筛选研究[J].中草药,2026,57(8):3121-3141,21.基金项目
河南中医药大学博士科研启动基金项目(00104311-2024) （00104311-2024）
河南省高等学校青年骨干教师培养计划(2021GGJS086) （2021GGJS086）
中央本级重大增减支项目"名贵中药资源可持续利用能力建设项目"(2060302) （2060302）
