中草药2026，Vol.57Issue(8)：3142-3153,12.DOI:10.7501/j.issn.0253-2670.2026.08.025
柴胡根部细菌群落的生态位富集特征与柴胡皂苷积累的关联分析
Correlation analysis of niche enrichment characteristics of root bacterial communities in Bupleuri genus and saikosaponins accumulation
摘要
Abstract
Objective To identify key bacterial taxa associated with saikosaponins content.Methods Rhizosphere soil,root surface soil,and root samples of Bupleurum chinense,B.falcatum,and B.scorzonerifolium were collected.Total DNA was extracted for metagenomic analysis,and UPLC-MS was used to determine the contents of saikosaponin A(SSA),saikosaponin B2(SSB2),saikosaponin B4(SSB4),saikosaponin C(SSC),saikosaponin D(SSD),saikosaponin E(SSE),and saikosaponin F(SSF)in the roots.Pearson correlation analysis was then employed to identify significant relationships between these medicinal components and the dominant bacterial species.Results SSD exhibited the highest content among the seven saikosaponins analyzed,with average concentrations of 217.5 μg/g in B.chinense,116.75 μg/g in B.falcatum,and 139.7 μg/g in B.scorzonerifolium.B.falcatum had the lowest content of SSE and SSF.Metagenomic sequencing identified a total of 5 440 bacterial OTUs.The numbers of unique OTUs in the root surface,rhizosphere,and endophytic compartments were 127,126,and 110 for B.chinense;91,74,and 69 for B.falcatum;and 43,137,and 126 for B.scorzonerifolium,respectively.Azotobacter chroococcum was dominant in the rhizosphere of B.chinense and B.scorzonerifolium,whereas Streptomyces was the core taxon in the rhizosphere of B.falcatum.Klebsiella sp.and Pseudomonas were significantly enriched in the root surface and endophytic niches of B.chinense and B.falcatum,respectively.The Klebsiella sp.,Cellulomonas sp.,and Azotobacter chroococcum were also a highly significant positive correlated with the contents of SSA,SSB2,SSB4,and SSD,among the correlation network analysis.Conclusion This study revealed that Bupleurum genotype might drive saikosaponins accumulation by niche construction of specific microbial communities.关键词
柴胡属/柴胡皂苷/根部细菌群落/宏基因组测序/植物-微生物互作/柴胡皂苷A/柴胡皂苷B2/柴胡皂苷B4/柴胡皂苷C/柴胡皂苷D/柴胡皂苷E/柴胡皂苷FKey words
Bupleuri genus/saikosaponins/root bacterial community/metagenomic sequencing/plant-microbe interaction/saikosaponin A/saikosaponin B2/saikosaponin B4/saikosaponin C/saikosaponin D/saikosaponin E/saikosaponin F分类
医药卫生引用本文复制引用
魏祯,陈华,莫传鑫,王田斌,师倩囡,闫明月,余马,赵军,辛超,陈文帅..柴胡根部细菌群落的生态位富集特征与柴胡皂苷积累的关联分析[J].中草药,2026,57(8):3142-3153,12.基金项目
西藏自治区重点研发计划(XZ202401ZY0020) （XZ202401ZY0020）
药用植物种质资源库建设(2021-I2M-1-032) （2021-I2M-1-032）
国家岗位产业体系专家项目(CARS-21) （CARS-21）
四川省区域创新合作项目(2026YFHZ0163) （2026YFHZ0163）
