西南农业学报2026，Vol.39Issue(3)：567-575,9.DOI:10.16213/j.cnki.scjas.2026.3.010
北方根结线虫分泌蛋白预测及特异性引物设计
Prediction of secreted protein and design of specific primers of Meloidogyne hapla
摘要
Abstract
[Objective]Meloidogyne hapla affects the yield and quality of Chinese medicinal materials.Predicting the secreted proteins of M.hapla and designing specific primers lay a foundation for the rapid and accurate identification of M.hapla.[Method]SignalP 6.0,TargetP-2.0,WoLF PSORT,ProtComp 9.0,DeepTMHMM1.0,big-PI predictor and dbCAN3 softwares were used to predict and analyze the secreted proteins and carbohydrate active enzymes(C AZymes)of the protein sequence of M.hapla,and specific primer design and validation were carried out based on the gene sequence encoding the specific secretory protein.[Result]Among the 14 433 protein sequences of M.hapla,498 protein sequences had the characteristics of typical secretory proteins,accounting for 3.45％of the total protein sequences.The length of secretory proteins was mainly distributed in 100-300 amino acids;the signal peptide length of secreted proteins was mainly distributed in 17-27 amino acids.A total of 17 CAZymes were predicted from 498 secreted proteins,including glycoside hydrolases(GHs),polysaccharide lyases(PLs)and carbohydrate binding modules(CBMs).The M.hapla specific primer Mhs-F2/R2 could effectively discriminate M.hapla from M.enterolobii.[Conclusion]A total of 498 proteins sequences with typical secretory protein characteristics were predicted,and the length characteristics,amino acid composition and signal peptide length of secretory proteins were analyzed.Specific primers were designed based on the DNA sequence of the specific secretory protein(A0A1I8BG71)of M.hapla,which could distinguish M.hapla from M.enterolobii.关键词
北方根结线虫/分泌蛋白预测/特异性引物设计/分子鉴定Key words
Meloidogyne hapla/Secretory proteome prediction/Specific primer design/Molecular identification分类
农业科技引用本文复制引用
先英豪,杨银智,马维思,李晓杨,杨斌,彭识,赵艳丽,杨永红..北方根结线虫分泌蛋白预测及特异性引物设计[J].西南农业学报,2026,39(3):567-575,9.基金项目
云南省科技厅科技计划项目(202305AR340003,202305AC160025) （202305AR340003,202305AC160025）
