中草药2026，Vol.57Issue(7)：2445-2455,11.DOI:10.7501/j.issn.0253-2670.2026.07.001
桔梗总皂苷肠道菌转化产物的化学成分及其抗药物性肝损伤活性研究
Chemical components of intestinal bacterial transformation products from total saponins of Platycodonis Radix and their anti-drug-induced liver injury activity
摘要
Abstract
Objective To investigate the chemical constituents of the intestinal bacteria transformation products of total saponins from Platycodon grandiflorus and their anti-drug-induced liver injury activity.Methods The compounds were isolated and purified by column chromatography techniques including AB-8 macroporous resin,normal phase silica gel,Sephadex LH-20 gel chromatography,and semi-preparative high-performance liquid chromatography.Their structures were identified based on physicochemical properties,mass spectrometry and nuclear magnetic resonance spectral data.The anti-drug-induced liver injury activities of the isolated compounds were evaluated using an acetaminophen-induced AML 12 cell hepatic injury model.Results A total of 16 compounds were isolated from the intestinal bacteria transformation products of total saponins from P.grandifloras and identified as 3-O-β-D-glucopyranosyloxy-2β,3β,16α,23-tetrahydroxyolean-12-en-28-oic acid-28-O-α-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranoside(1),polygalacin D(2),3-O-β-D-glucopyranosyl platycodigenin(3),platycodin D3(4),platycoside F(5),platycodin D(6),deapio-platycodin D(7),platycodonoid B(8),3-O-β-D-glucopyranosyl polygalacic acid(9),leiyemudanoside A(10),linoleic acid(11),(6Z,9Z)-6,9-hexadecadienoic acid(12),glyceryl linoleate(13),(2S)-1-O-(9Z,12Z-octadecandienyl)-3-O-β-galactosyl glycerol(14),dibutyl phthalate(15),and triallyl isocyanurate(16).Anti-drug-induced liver injury activity study showed that compounds 1-3,5-7,9,and 14 significantly inhibited the levels of alanine aminotransferase and aspartate aminotransferase in acetaminophen-induced AML 12 cell supernatants(P＜0.05,0.01).Conclusion A total of 16 compounds were successfully isolated and identified from the intestinal bacterial transformation products from total saponins of P.grandiflorus.Compound 1 is a new compound named des-apio-xylosyl polygalacin D.Compounds 1-3,5-7,9,and 14 exhibit significant anti-drug-induced liver injury activities,showing potential hepatoprotective effects.关键词
桔梗总皂苷/肠道菌/转化产物/药物性肝损伤/去芹糖木糖基远志皂苷D/远志皂苷D/桔梗皂苷D3Key words
total saponins of Platycodonis Radix/intestinal bacterial/transformation product/drug-induced liver injury/des-apio-xylosyl polygalacin D/polygalacin D/platycodin D3分类
医药卫生引用本文复制引用
彭昱,钟国跃,曾金祥,梁永红,钟渊涵,万同林,刘光鹏,文丽云,饶建波,梁健,秦倩,张寿文..桔梗总皂苷肠道菌转化产物的化学成分及其抗药物性肝损伤活性研究[J].中草药,2026,57(7):2445-2455,11.基金项目
国家自然科学基金项目(82160736) （82160736）
江西中医药大学科技创新团队项目(CXTD22002) （CXTD22002）
