油气田环境保护2026，Vol.36Issue(2)：29-34,6.DOI:10.3969/j.issn.1005-3158.2026.02.005
生物絮凝剂产生菌的筛选鉴定及在页岩油污水处理中的应用
Screening and Identification of a Bioflocculant-Producing Strain and Its Application in Shale-Oil Wastewater Treatment
摘要
Abstract
A total of 56 bacterial strains were isolated from oilfield soil and secondary sedimentation tank sludge,among which 12 showed significant flocculation activity.Through secondary screening,a highly efficient bioflocculant-producing strain was obtained and identified as Bacillus cereus using molecular biological techniques.To enhance its activity,culture conditions-including carbon and nitrogen sources,pH,rotation speed,temperature,and inoculum size-were optimized.Single-factor experimental results demonstrated that under weakly alkaline conditions,the shale oil removal rate of this bioflocculant was 8.8%to 19.3%higher than that of polyacrylamide(PAM).Moreover,the bioflocculant achieved superior removal efficiency for both turbidity and oil content in shale oil wastewater.关键词
生物絮凝剂/蜡样芽孢杆菌/页岩油污水/含油量/絮凝活性/培养条件优化Key words
microbial flocculant/Bacillus cereus/shale oil wastewater/oil content/flocculating activity/optimization of culture conditions分类
资源环境引用本文复制引用
刘鑫磊,齐晗兵,张宇新,张晓雪..生物絮凝剂产生菌的筛选鉴定及在页岩油污水处理中的应用[J].油气田环境保护,2026,36(2):29-34,6.基金项目
大庆市指导项目《页岩油污水厌氧处理工艺中挥发性脂肪酸光学检测机理研究》(zd-2023-43). （zd-2023-43）
