中国畜牧杂志2026，Vol.62Issue(5)：108-114,7.DOI:10.19556/j.0258-7033.20250304-07
酵母表面展示技术在饲用酶研究中的应用
Application of Yeast Surface Display Technology in the Reseach of Feed Enzymes
摘要
Abstract
Yeast surface display(YSD)is a whole-cell catalytic technology that achieves immobilized presentation of functional enzymes on the yeast cell surface.This is accomplished by fusing heterologous functional enzymes with yeast cell wall proteins and leveraging natural intracellular transport mechanisms(such as glycosylphosphatidylinositol(GPI)-anchored system and protein with internal repeats(Pir)-protein system,etc.)to transport these fusion proteins to the cell membrane surface.Traditional enzyme preparations suffer from high separation and purification costs,low product reuse rates,and poor stability.In contrast,whole-cell catalysts produced using YSD technology require no additional purification or immobilization steps.Compared to free enzymes,whole-cell catalysts also exhibit superior stability and can be reused multiple times.In order to facilitate the researchers in relevant fields to understand the current research status and application potential of YSD technology in the field of feed enzymes,this review firstly introduces the types and functions of common feed enzymes and yeast cell wall-anchored proteins;secondly,it compares and summarizes the advantages of yeast surface-displayed feed enzymes over traditional free enzymes in terms of enzymatic properties and applications;and finally,it analyzes the problems of YSD for feed enzymes and possible research directions in the future.关键词
饲用酶/酵母表面展示技术/全细胞催化/锚定蛋白/应用Key words
Feed enzyme/Yeast surface display technology/Whole-cell catalysis/Anchoring protein/Application分类
农业科技引用本文复制引用
郑智勇,胡凯文,金三俊,张梦捷,李国喜,尹清强,李新新..酵母表面展示技术在饲用酶研究中的应用[J].中国畜牧杂志,2026,62(5):108-114,7.基金项目
河南省科技研发计划联合基金(应用攻关类)项目(242103810001) （应用攻关类）
畜禽营养与饲养全国重点实验室开放课题(2004DA125184H2506) （2004DA125184H2506）
农业农村部饲料生物技术重点实验室开放课题资助或部分资助 （）
河南省科技攻关项目(242102110058) （242102110058）
河南农业大学动物科技学院大学生科技创新项目(2024DXSKC23) （2024DXSKC23）
河南农业大学大学生创新训练计划项目 （）
河南农业大学本科教育教学改革研究与实践项目(2025XJGLX130) （2025XJGLX130）
