中华中医药杂志2026，Vol.41Issue(3)：955-960,6.
化瘀祛痰方对ApoE-/-动脉粥样硬化小鼠主动脉巨噬细胞M1极化及糖酵解相关途径的影响
Effects of Huayu Qutan Formula on M1 polarization and glycolysis related pathways of aortic macrophages in ApoE-/-atherosclerosis mice
摘要
Abstract
Objective:To investigate the molecular mechanism by which the Huayu Qutan Formula inhibits the progression of atherosclerosis(AS)by regulating macrophage M1 polarization and related glycolysis pathways in the aortas of ApoE-/-AS mice.Methods:A total of 24 ApoE-/-mice were randomly divided into three groups:model group,Huayu Qutan Formula group,and rosuvastatin group,they were fed a high-fat diet,while the 8 C57BL/6J mice were fed a standard diet as the normal control group.After 8 weeks of dietary intervention,Huayu Qutan Formula group and rosuvastatin group were administered their respective treatments via gavage for 4 weeks.Serum lipid levels were measured using an automated biochemical analyzer.Pathological changes in the aortic tissues were observed through HE and oil red O staining.Levels of IL-1β,IL-6,IL-12,LDH,and TNF-α in serum and aortic tissues were detected by ELISA.Co-localization of LDH and HK2 in the aorta was examined using immunofluorescence.mRNA and protein expression levels of GLUT1,HK2,PKM2,PFK,and HIF-1α in the aorta were assessed by RT-qPCR and Western Blot.Serum levels of G6PD and LA were measured using a microplate method.Results:Compared with the model group,both the Huayu Qutan Formula group and the rosuvastatin group exhibited significantly reduced serum levels of TC,TG,and LDL-C(P＜0.01)and increased HDL-C(P＜0.01),atherosclerotic plaques were significantly reduced,and co-localization of CD86(green),CD80(red),and LDH(pink),as well as HK2(pink),was markedly decreased,levels of IL-1β,IL-6,IL-12,LDH,and TNF-α in serum and aortic tissues were significantly lowered(P＜0.01),mRNA and protein expression of GLUT1,HK2,PKM2,PFK and HIF-1α in the aorta were significantly downregulated(P＜0.01),serum levels of G6PD and LA were also significantly reduced(P＜0.01).Conclusion:Huayu Qutan Formula exerts anti-atherosclerotic effects by improving dyslipidemia,modulating macrophage glycolysis pathways,influencing macrophage polarization,and inhibiting the production of pro-inflammatory cytokines.关键词
巨噬细胞/糖酵解/化瘀祛痰方/动脉粥样硬化/机制Key words
Macrophage/Glycolysis/Huayu Qutan Formula/Atherosclerosis(AS)/Mechanism引用本文复制引用
崔运浩,贾连群..化瘀祛痰方对ApoE-/-动脉粥样硬化小鼠主动脉巨噬细胞M1极化及糖酵解相关途径的影响[J].中华中医药杂志,2026,41(3):955-960,6.基金项目
辽宁省科技计划联合计划(应用基础研究项目)(No.2023JH2/101700203),辽宁省科技计划联合计划(技术攻关计划项目)(No.2024JH2/102600194) （应用基础研究项目）
