中国中医眼科杂志2026，Vol.36Issue(5)：426-433,8.DOI:10.13444/j.cnki.zgzyykzz.2026.05.005
基于网络药理学探讨逍生散治疗干眼的作用机制
Exploring the Mechanism of Action of Xiaosheng Powder in Treating Dry Eye Based on Network Pharmacology
摘要
Abstract
OBJECTIVE To investigate the mechanism of action of Xiaosheng Powder in treating dry eye based on network pharmacology and molecular docking technology.METHODS The active components and related targets of the medicine components of Xiaosheng Powder were retrieved using the TCMSP and medicine databases.The Swiss Target Prediction platform was used to predict targets of the effective compounds.Dry eye-related targets were obtained by searching the GeneCards,OMIM,TTD,PharmGkb,and DrugBank databases.A Venn diagram was constructed to obtain the medicine-disease intersection data.The intersecting genes were uploaded to the STRING platform to construct a protein-protein interaction(PPI)network.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were performed on the intersecting targets.Molecular docking verification was conducted between the obtained core targets and the active components with high degree values in the regulatory network.RESULTS(1)Active components and targets:A total of 718 active components of Xiaosheng Powder were screened,with 817 corresponding targets.Intersection with 4,498 dry eye-related disease target genes yielded 445 common"medicine active component-disease"target genes.(2)Construction of the medicine-component-target-disease network:The medicine-component-target-disease network diagram included 550 nodes and 2,042 edges.(3)PPI network:The PPI network of the common targets contained 376 nodes and 3,010 edges.The top three targets by Degree value were tumor protein p53(TP53),signal transducer and activator of transcription 3(STAT3),and proto-oncogene tyrosine-protein kinase Src(SRC).(4)GO and KEGG pathway enrichment analyses:Biological processes were mainly concentrated in the positive regulation of the mitogen-activated protein kinase(MAPK)cascade,response to external stimuli,and positive regulation of kinase activity,etc.Cellular components were mainly concentrated in membrane rafts,membrane microdomains,and neuronal cell bodies,etc.Molecular functions were mainly concentrated on serine/threonine kinase activity,etc.Key pathways mainly included the phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)and MAPK signaling pathways.(5)Molecular docking:The core targets and the main active components all exhibited strong binding affinities.CONCLUSIONS The core targets of Xiaosheng Powder in treating dry eye involve signaling pathways such as PI3K/AKT and MAPK,and its active components show good binding activity with these core targets.This study elucidates the molecular-level mechanism of action of this formula in treating dry eye.关键词
逍生散/作用机制/网络药理学/PI3K/AKTKey words
Xiaosheng Powder/mechanism of action/network pharmacology/PI3K/AKT分类
医药卫生引用本文复制引用
李晓宇,谢立科,郝晓凤,秦睿,刘亮,鞠品..基于网络药理学探讨逍生散治疗干眼的作用机制[J].中国中医眼科杂志,2026,36(5):426-433,8.基金项目
1 国家自然科学基金项目(82174445、82405491)2 中国中医科学院眼科医院高水平中医院揭榜挂帅项目(GSP4-02)3 中国中医科学院眼科医院新入职青年科研人员培养专项(XJ2023001302) （82174445、82405491）
