种子2026，Vol.45Issue(3)：22-29,8.DOI:10.16590/j.cnki.1001-4705.2026.03.022
菘蓝果实外果皮对自身种子萌发抑制作用及生理机制研究
Study on the Inhibitory Effect and Physiological Mechanisms of the Exocarp of Isatis indigotica Fruits on the Germination of Their Own Seeds
摘要
Abstract
To explore the mechanism of the effect of the extract from the Isatis tinctoria fruit on the seed ger-mination,based on the water absorption characteristics and viability of the Isatis tinctoria siliques and seeds,the extraction time for the preparation of the Isatis tinctoria fruit extract was determined.Using non-target metabolite detection,water-soluble differential metabolites were screened,and through exogenous application experiments,the effects of this substance and the original extract on the physiological activity of Isatis tincto-ria seeds in the early stage(0-3 days)of germination were analyzed.The results showed that the Isatis tinc-toria siliques soaked for 20 hours reached the state of seed swelling,with the maximum water absorption rate of 291％,while the Isatis tinctoria seeds reached the state of swelling after soaking for 6 hours,with the maximum water absorption rate of 60.5％.The control group had a germination rate of 93.67％,the silique extract group had a germination rate of 6.67％,and the Isatis tinctoria seed extract group had a germination rate of 69.33％.163 differential metabolites with|log2(FC)|≥5 were screened,among which 41 were wa-ter-soluble differential metabolites.Exogenous application of 3-hydroxy-2-pyridinecarboxylic acid had a strong inhibitory effect on Isatis tinctoria seed germination.The exogenous application of the extract and 3-hydroxy-2-pyridinecarboxylic acid solution affected the changes in the contents and activities of starch,SBE,sucrose,sucrosease,POD,soluble protein,GA,IAA and ABA in Isatis tinctoria seeds.The study showed that the Isatis tinctoria silique extract had an inhibitory effect on its own seed germination,and it was the combined effect of multiple substances.The inhibitors were mainly concentrated in the exocarp of the Isatis tinctoria si-lique and the inhibitors regulated seed germination by affecting the metabolism of substances such as starch,soluble protein,sucrosease,SBE and IAA in Isatis tinctoria seeds.关键词
菘蓝角果/种子萌发/浸提液/抑制作用/代谢组Key words
Isatis tinctoria silique/seed germination/extract/inhibitory effect/metabolome分类
农业科技引用本文复制引用
刘东,孟瑾瑾,赵强,久西加,徐圆圆,陈红刚,杜弢,王盼..菘蓝果实外果皮对自身种子萌发抑制作用及生理机制研究[J].种子,2026,45(3):22-29,8.基金项目
国家中药材产业技术体系建设专项资金(CARS-21) （CARS-21）
甘肃省教育厅高校教师创新基金项目(2025A-406) （2025A-406）
甘肃省研究生"创新之星"项目/甘肃中医药大学研究生"创新创业基金"(2025CXZX-924) （2025CXZX-924）
