种子2026，Vol.45Issue(3)：30-37,8.DOI:10.16590/j.cnki.1001-4705.2026.03.030
高羊茅FabHLH63基因克隆及在干旱胁迫下表达分析
Cloning of the FabHLH63 Gene of Festuca elata and Analysis on Its Expression under Drought Stress
摘要
Abstract
In order to cultivate high-yielding and drought-resistant new varieties of Festuca elata,the RACE technology was used to screen and clone FabHLH63 from the drought transcriptome of Festu-ca elata.Bioinformatics,subcellular localization and expression analysis under drought stress were conducted.The results showed that the complete cDNA sequence of the FabHLH63 transcription fac-tor was 1 311 bp,encoding a protein containing 359 amino acid residues,with a typical bHLH do-main.The phylogenetic analysis revealed that it had a closer genetic relationship with the bHLH pro-tein of the grass species Lolium multiflorum.The subcellular localization indicated that the FabHLH63 protein was located in the nucleus.The results of real-time fluorescent quantitative PCR showed that the expression of FabHLH63 was significantly upregulated under drought stress,indica-ting that FabHLH63 could play an important role in the drought response.The research results pro-vided a theoretical basis for in-depth understanding of the molecular mechanism of FabHLH63 in the drought resistance of Festuca elata,and offerred potential gene targets for molecular breeding of Festuca elata.关键词
FabHLH63基因/克隆/生物信息学/亚细胞定位Key words
FabHLH63 gene/cloning/bioinformatics analysis/subcellular localization分类
农业科技引用本文复制引用
陈锡,龙显英,朱必达,赵德刚,王小利..高羊茅FabHLH63基因克隆及在干旱胁迫下表达分析[J].种子,2026,45(3):30-37,8.基金项目
贵州省农业科学院开放基金项目(黔农科开放基金[2024]01号)、贵州省科技计划项目(黔科合基础-ZK[2022]一般222) （黔农科开放基金[2024]01号）
贵州省育种科研基础平台创新能力建设(黔科合服企[2022]014) （黔科合服企[2022]014）
喀斯特山区特色作物生物育种创新能力建设(黔科合服企[2024]003-1)和喀斯特山区特色粮油作物生物育种部省共建重点实验室科研能力条件建设(黔科合服企[2024]003-2) （黔科合服企[2024]003-1）
