中国药理学通报2026，Vol.42Issue(4)：644-651,8.DOI:10.12360/CPB202503121
DADS上调miR-7通过EGFR/Rac1/Pak1信号通路抑制胃癌SGC-7901细胞增殖、迁移侵袭与EMT
DADS up-regulates miR-7 to inhibit proliferation,migration,invasion and EMT of gastric cancer SGC-7901 cells through EGFR/Rac1/Pak1 signaling pathway
摘要
Abstract
Aim To investigate the inhibition of EMT migration and invasion of SGC-7901 cells by DADS down-regulation of miR-7 through EGFR/Rac1/Pak1 pathway and its molecular mechanism.Methods Lentivirus PHBLV-U6-RFP-T2A-Puro-HSA-Mir-7-an-tago and control empty vector pHBLV-U6-RFP-T2A-Puro were transfected with SGC-7901 to establish a stable Mir-7-silenced SGC-7901 cell line.MTT,scratch,migration and invasion assay were used to de-tect cell proliferation,migration and invasion.qRT-PCT,Western blot and immunofluorescence were used to detect the expressions of EGFR,Rac1,Pak1,Vi-mentin and E-cadherin.Results SGC-7901 cells with stable silencing of miR-7 were successfully con-structed.qRT-PCR showed that the expression of miR-7 in SGC7901 transfected with lentivirus si-mir7 was significantly down-regulated.The expression of miR-7 in SGC-7901 cells was significantly up-regulated after DADS treatment.After 24 h,48 h and 72 h,the pro-liferation rate of miR-7 silencing group significantly in-creased compared with SGC7901 group and vector group.The growth rate of DADS after treatment was lower than that before treatment.The proliferation rate of Mir-7-silenced+DADS group was lower than that of SGC-7901 and vector groups.The scratch distance of miR-7 silencing group was significantly shorter than that of SGC7901 group and vector group.The scratch-ing distance of the cells after DADS treatment was wider than that before DADS treatment.The invasion cells of miR-7 silencing group significantly increased compared with SGC7901 group and vector group.The invasion of DADS cells in all groups significantly de-creased after DADS treatment,and the miR-7 silenc-ing+DADS group significantly decreased.EGFR,Rac1,Pak1 and Vimentin were up-regulated and E-cadherin was down-regulated in miR-7 silencing group compared with SGC7901 group and vector group.After DADS treatment,EGFR,Rac1,Pak1 and Vi-mentin were down-regulated and E-cadherin was up-regulated in all groups,with the most significant changes observed in the miR-7 silence group.Conclu-sion DADS can up-regulate miR-7 to inhibit the pro-liferation,EMT,migration and invasion of SGC-7901 cells through EGFR/Rac1/Pak1 pathway,and miR-7 silences play a role in promoting it.关键词
二烯丙基二硫/胃癌SGC-7901细胞/miR-7/EGFR/Rac1/Pak1信号通路/EMT/迁移侵袭Key words
diallyl disulfide/gastric cancer SGC-7901 cells/miR-7/EGFR/Rac1/Pak1 path/EMT/mi-gration and invasion分类
医药卫生引用本文复制引用
朱欢,邓玲,陈洁海,刘芳,伍尤华,苏琦..DADS上调miR-7通过EGFR/Rac1/Pak1信号通路抑制胃癌SGC-7901细胞增殖、迁移侵袭与EMT[J].中国药理学通报,2026,42(4):644-651,8.基金项目
国家自然科学基金资助项目(No 81973532) （No 81973532）
湖南省卫计委科研重点课题(No A2015-2) （No A2015-2）
湖南省高校创新平台开放基金(No 17K081) （No 17K081）
