浙江农业学报2026，Vol.38Issue(4)：632-642,11.DOI:10.3969/j.issn.1004-1524.20250304
与传染性支气管炎病毒核衣壳蛋白互作的宿主RNA结合蛋白的鉴定
Identification of host RNA-binding proteins interacting with infectious bronchitis virus N protein
摘要
Abstract
To screen host RNA-binding proteins interacting with the infectious bronchitis virus(IBV)N protein,providing a basis for elucidating the virus-host interaction mechanism,co-immunoprecipitation(Co-IP)combined with mass spectrometry was employed to identify host proteins interacting with the N protein with the monoclonal anti-body 4H4 against IBV N protein.Differentially expressed proteins were subjected to bioinformatics analysis,and Co-IP experiments were conducted to validate the interactions between the N protein and candidate RNA-binding pro-teins.The results showed that a total of 847 differentially expressed proteins were identified,primarily involved in processes such as transcription,translation and protein modification,protein-RNA complex assembly,signal trans-duction,and energy metabolism.Co-IP experimental results indicated that interactions exist between the N protein and the host RNA-binding proteins TARDBP,hnRNPK,FUBP3,and hnRNPA1.This study identified host proteins interacting with the IBV N protein,which are involved in multiple key stages of the viral replication cycle.The inter-actions between the N protein and RNA-binding proteins provide new research directions for elucidating the specific mechanisms of host proteins in viral infection.关键词
传染性支气管炎病毒/N蛋白/RNA结合蛋白/病毒-宿主互作/免疫共沉淀/单克隆抗体Key words
infectious bronchitis virus/N protein/RNA-binding protein/virus-host interaction/co-immunoprecipi-tation/monoclonal antibody分类
农业科技引用本文复制引用
代雪艳,肖鹏,符雪珍,胡洁,常宇昕,赵伟,廖敏..与传染性支气管炎病毒核衣壳蛋白互作的宿主RNA结合蛋白的鉴定[J].浙江农业学报,2026,38(4):632-642,11.基金项目
浙江省基础公益计划项目(LGN22C18003) （LGN22C18003）
浙江省"三农九方"科技协作项目(2024SNJF046) （2024SNJF046）
