浙江农业学报2026，Vol.38Issue(4)：669-679,11.DOI:10.3969/j.issn.1004-1524.20250337
pH值与离子效应对草菇生物量与IAA合成的调控
Regulation of biomass and IAA synthesis in Volvariella volvacea by pH value and ionic effects
摘要
Abstract
Exogenous sodium acetate(NaAc)can promote indole-3-acetic acid(IAA)synthesis during the primor-dium stage of Volvariella volvacea,thereby promoting primordium differentiation and increasing yield,but its specific mechanism of action has not been elucidated.This study aimed to analyze the ability of NaAc to induce IAA synthe-sis.Using a submerged culture method,a three-factor experiment was designed with five pH value gradients(6,7,8,9,10),four anions(Ac-,HCO3-,C5H7O5COO-,H2PO4-),and four cations(Na+,Mg2+,Mn2+,K+)to investigate the effects of adding NaAc on mycelial biomass and IAA synthesis,providing a theoretical basis for sub-sequently revealing the mechanism by which NaAc regulates IAA synthesis.The results showed that under pH value 8 conditions,mycelial biomass,aldehyde dehydrogenase(ALDH)activity,and the contents of IAA,indole-3-acet-aldehyde(IAAld),indole-3-pyruvic acid(IPyA),tryptamine(TAM)and indole-3-acetaldoxime(IAOx)were sig-nificantly(p＜0.05)increased by 37.98％,58.39％,61.29％,58.33％,31.25％,29.23％and 64.71％,re-spectively.Among the anions,acetate ions(Ac-)significantly increased biomass,IAA content,and ALDH activity by 6.28％,22.58％,and 21.01％,respectively,the contents of IPyA,TAM,and IAOx were also significantly higher than the control.Among the cations,Na+significantly increased biomass,IAA content,and ALDH activity by 6.28％,22.58％and 20.97％,respectively,and the contents of IPyA,TAM,and IAOx were also significantly higher than the control.This indicated that Na+was superior to Mg2+,Mn2+,and K+in promoting IAA synthesis.Comprehensive analysis showed that during the vegetative growth stage of V.volvacea,adding NaAc could maintain the culture environment pH value within an alkaline range suitable for growth.pH value,as the main regulatory fac-tor after NaAc dissociation,together with Ac-and Na+,influenced biomass accumulation and IAA synthesis during the growth stage of V.volvacea.关键词
草菇/pH值/离子效应/IAA合成/生物量Key words
Volvariella volvacea/pH value/ionic effect/IAA synthesis/biomass分类
农业科技引用本文复制引用
卯明娟,刘迪,普柒牛,侯立娟,马林,蒋宁,林金盛,曲绍轩,李辉平,徐平..pH值与离子效应对草菇生物量与IAA合成的调控[J].浙江农业学报,2026,38(4):669-679,11.基金项目
江苏省农业科技自主创新基金项目[CX(22)3141] （22）
国家自然科学基金青年科学基金(31901933) （31901933）
