浙江农业学报2026，Vol.38Issue(4)：696-706,11.DOI:10.3969/j.issn.1004-1524.20250254
绣球博大蓝叶斑病病原菌的分离鉴定与防治
Isolation,identification,and control of the pathogen causing leaf spot disease on Hydrangea macrophylla Bo Dalan
摘要
Abstract
To identify the pathogen causing leaf spot disease on Hydrangea macrophylla Bo Dalan and investigate its biological characteristics and effective control agents,thereby providing a theoretical basis for the scientific manage-ment of this disease,this study isolated the pathogen from diseased samples collected at the Nanguo Shanhua Hydran-gea Germplasm Resource Base in Yuxi City,Yunnan Province,using the tissue isolation method.The pathogenicity was verified according to Koch's postulates.The pathogen was identified based on morphological observations and multi-gene sequence(ITS,LSU,TUB)analysis.The effects of different culture media,carbon sources,nitrogen sources,temperature,light conditions,and pH value on the mycelial growth of the pathogen were studied using the mycelial growth rate method.Additionally,nine commonly used fungicides were selected to determine their in vitro toxicity against the pathogen.The results showed that the primary pathogen isolated from the infected leaves was Epi-coccum sorghinum.The biological characteristics tests indicated that the optimal conditions for mycelial growth were PDA medium with maltose as the carbon source and peptone and beef extract as nitrogen sources,at a temperature of 25 ℃,pH value 7.0,and under continuous light.The in vitro toxicity tests revealed that 10％difenoconazole water dispersible granules(WG)had the best inhibitory effect,with an EC50 value of 1.252 2 mg·L-1.In conclusion,the pathogen causing leaf spot disease on H.macrophylla Bo Dalan was E.sorghinum,and 10％difenoconazole WG ex-hibited a strong inhibitory effect against this pathogen,making it a potential candidate for field control.关键词
绣球博大蓝/叶斑病/病原菌/高粱附球菌/生物学特性/10％苯醚甲环唑水分散性粒剂Key words
Hydrangea Bo Dalan/leaf spot disease/pathogen/Epicoccum sorghinum/biological characteristic/10％difenoconazole water dispersible granules(WG)分类
农业科技引用本文复制引用
张玉,陈晶晶,温联好,杨振翼,杨曾华,童译,王超..绣球博大蓝叶斑病病原菌的分离鉴定与防治[J].浙江农业学报,2026,38(4):696-706,11.基金项目
云南省重大科技专项(202202AE090028) （202202AE090028）
云南省大学生创新训练计划项目(20200554052) （20200554052）
