植物保护学报2026，Vol.53Issue(2)：356-366,11.DOI:10.13802/j.cnki.zwbhxb.2026.2025154
番茄晚疫病菌拮抗菌株筛选鉴定及其作用机制
Isolation,identification of antagonistic bacterial strains,and the antagonistic mechanisms against tomato late blight pathogen
摘要
Abstract
To screen microbial resources for the control of tomato late blight caused by pathogen Phy-tophthora infestans,strains with strong antagonistic activity were isolated from soil using the plate con-frontation method.Their taxonomic status was determined using integrating morphological,physiologi-cal,and biochemical characteristics,combined with the 16S rDNA gene sequence analysis.The antifun-gal activity,action mechanisms,and control effect of the antagonistic strains were investigated via the mycelial growth inhibition method,spore germination assay,and pot experiment.The results showed that a total of 11 strains exhibited antagonistic activities against P.infestans.Among these antagonistic strains,strain C-21 exhibited the highest mycelial growth inhibition rate of 84.41%,and it was identi-fied as Streptomyces flavogriseus.This strain could produce extracellular enzymes including cellulase,protease and amylase.Its fermentation broth could disrupt the mycelial morphology and cell membrane integrity of P.infestans,and significantly inhibited spore germination and zoospore release,with inhibi-tion rates of 69.31%and 57.94%,respectively.The preventive and curative control effects of strain C-21 against tomato late blight were 43.57%and 59.28%,respectively.These findings indicated that strain C-21 had an excellent control effect on tomato late blight,and exhibited promising biocontrol potential for further development and application.关键词
番茄晚疫病/致病疫霉/黄灰链霉菌/生物防治/作用机制Key words
tomato late blight/Phytophthora infestans/Streptomyces flavogriseus/biological control/action mechanism引用本文复制引用
彭佳乐,杨平鸽,董欣杰,刘书剑,王磊,姚艳平,郝晓娟,王美琴..番茄晚疫病菌拮抗菌株筛选鉴定及其作用机制[J].植物保护学报,2026,53(2):356-366,11.基金项目
山西省现代农业产业技术体系建设项目(2025CYJSTX08),山西省基础研究计划(自由探索类)面上项目(20210302123404) （2025CYJSTX08）
