植物保护学报2026，Vol.53Issue(2)：367-382,16.DOI:10.13802/j.cnki.zwbhxb.2026.2025115
宁夏酿酒葡萄根腐病病原鉴定及其拮抗菌株筛选
Identification of grapevine root rot and screening of antagonistic bacterial strains in Ningxia wine grape growing region
摘要
Abstract
To identify the pathogens causing grapevine root rot in Ningxia Hui Autonomous Region(hereinafter referred to as Ningxia)and to explore indigenous antagonistic microorganisms for develop-ing biocontrol agents,grapevine samples with root rot symptoms were collected from representative winery vineyards in five cities and counties of Ningxia.The pathogens were identified through isola-tion,identification and pathogenicity tests.Using the more virulent pathogens as indicator strains and the wine grape cultivar Cabernet Sauvignon as the experimental material,the biocontrol efficacy of bac-teria isolated from the rhizosphere soil of healthy grapevines was evaluated through plate confrontation assays,mycelial growth inhibition tests and pot experiments.The results showed that the pathogens causing grapevine root rot in Ningxia were Fusarium acuminatum,Fusarium solani,Rhizoctonia solani,Neofusicoccum parvum and Lasiodiplodia theobromae,among which R.solani,N.parvum and L.theo-bromae are new records as pathogens of grapevine root rot.Fusarium acuminatum,R.solani,N.parvum and L.theobromae exhibited stronger pathogenicity.A total of 62 bacterial and actinomycete strains with distinct colony morphologies were isolated from the rhizosphere soil of healthy grapevines,and four strains exhibiting over 60%inhibitory activity against all four highly pathogenic fungi were selected,namely Bacillus velezensis,Streptomyces alfalfae,Amycolatopsis orientalis and Streptomyces griseus.Among these,the 10.0%sterile culture filtrate of B.velezensis exhibited the strongest inhibitory effect against the four pathogens tested,with inhibition rates ranging from 80.56%to 84.65%,and its volatile compounds exhibited the strongest inhibitory effect against L.theobromae,with an inhibition rate of 73.67%.The three antagonistic actinomycetes showed differential inhibitory effects against dif-ferent pathogens.Inoculation with the four antagonistic strains via wounded root irrigation inhibited grapevine root rot to varying degrees and promoted an increase in root branch number,indicating that these four antagonistic strains exhibit significant growth-promoting and disease-suppressing effects and possess potential for development as biocontrol agents against grape root rot.关键词
葡萄根腐病/病原鉴定/拮抗细菌/拮抗放线菌/抑制作用/生物防治/宁夏Key words
grapevine root rot/pathogen identification/antagonistic bacteria/antagonistic actinomy-cetes/inhibitory effect/biological control/Ningxia引用本文复制引用
辛颖,赵楠,刘冠兰,蒲占悦,王一冬,顾沛雯..宁夏酿酒葡萄根腐病病原鉴定及其拮抗菌株筛选[J].植物保护学报,2026,53(2):367-382,16.基金项目
宁夏重点研发计划项目(2023BCF01026,2024BBF02006) （2023BCF01026,2024BBF02006）
