植物保护学报2026，Vol.53Issue(2)：394-408,15.DOI:10.13802/j.cnki.zwbhxb.2026.2025101
甜菜碱对稻瘟病的防效及其诱导水稻抗瘟性的分子机制
Control effect of betaine against rice blast and its molecular mechanism of inducing rice blast resistance
摘要
Abstract
To elucidate the control effect of the rice metabolite betaine on rice blast and its resistance-inducing mechanism,exogenous betaine sprays at different concentrations and time points were applied to assess the resistance of rice cultivars against rice blast.The optimal concentration and timing for exogenous betaine application were screened.Furthermore,transcriptome sequencing was performed on rice treated with betaine under the optimal application conditions to analyze its resistance induction mechanism.The results showed that exogenous application of betaine effectively reduced the severity of rice blast in the susceptible rice varieties Huangkenuo and Lijiangxintuanheigu.The optimal relative control effect(40.06%)was achieved with a pretreatment of 20 mmol/L betaine applied 24 hours before inoculation with Magnaporthe oryzae.Transcriptome analysis of rice leaves pretreated with betaine indicated that differentially expressed genes were significantly enriched in the phenylpropanoid biosyn-thesis pathway at 0,24,and 48 hours.This treatment also induced the expression of lignin synthesis-related genes,including OsCCR17,OsCCR14,OsPAL3,OsCOMT15,OsCAD2,and the peroxidase gene OsPOX1.Additionally,lignin content and the activities of peroxidase and superoxide dismutase were significantly increased.These findings suggest that betaine induces the expression of genes involved in lignin synthesis and antioxidant enzymes,promotes lignin accumulation,and enhances antioxidant enzyme activity,thereby improving systemic resistance in rice against rice blast.关键词
稻瘟病/甜菜碱/转录组/木质素/抗氧化酶活性Key words
rice blast/betaine/transcriptome/lignin/antioxidant enzyme activity引用本文复制引用
李荣萍,唐敏,刘金汪,王云月,何平,韩光煜..甜菜碱对稻瘟病的防效及其诱导水稻抗瘟性的分子机制[J].植物保护学报,2026,53(2):394-408,15.基金项目
云南省兴滇英才教育人才项目(XDYC-JYRC-2023-0094),国家自然科学基金地区基金项目(32260711),云南省基础研究计划面上项目(202501AT070156) （XDYC-JYRC-2023-0094）
