中西医结合肝病杂志2026，Vol.36Issue(4)：451-455,5.DOI:10.3969/j.issn.1005-0264.2026.004.011
山仙颗粒含药血清通过Wnt/β-catenin信号通路抑制肝卵圆细胞EMT及恶性转化相关表型
Shanxian granule-containing serum inhibits epithelial-mesenchymal transition(EMT)and malignant transformation of hepatic oval cells via the Wnt/β-catenin signaling pathway
摘要
Abstract
Objective:This study aimed to investigate the mechanism by which Shanxian Granule medicated serum(SXG)reverses epithelial-mesenchymal transition(EMT)and malignant transformation of the hepatic oval cell line WB-F344 through the Wnt/β-catenin signaling pathway.Methods:The malignant transformation and EMT model were induced in WB-F344 cells using N-methyl-N'-nitro-N-nitrosoguanidine(MNNG).Experimental groups were treated with low-,medium-,and high-dose SXG,while control groups included a normal group(blank serum),a model group(MNNG+blank serum),and a pathway validation group(high-dose SXG+Wnt activator CP21R7).Cell proliferation was assessed via MTT assay;migration and invasion abilities were evaluated using scratch wound healing and Transwell assays.Western blotting was employed to detect EMT-related markers(E-cadherin,N-cadherin,Vimentin),malignant transformation-associated proteins(cleaved Caspase-3,P53,CD34),and key molecules of the Wnt pathway(Wnt3a,β-catenin,C-myc).Results:Compared with the normal group,the model group exhibited significantly enhanced cell proliferation,migration,and invasion(P＜0.05),accompanied by downregulated E-cadherin,cleaved Caspase-3,and P53 protein levels,and upregulated N-cadherin,Vimentin,CD34,Wnt3a,β-catenin,and C-myc expression(P＜0.05).SXG intervention reversed these malignant phenotypes and EMT progression in a dose-dependent manner.However,the addition of CP21R7 antagonized the effects of SXG(P＜0.05).Conclusion:SXG inhibits EMT and malignant transformation of WB-F344 cells by suppressing the Wnt/β-catenin signaling pathway.关键词
山仙颗粒/上皮间质转化/恶性表型/癌前病变/肝卵圆细胞/Wnt/β-catenin信号通路Key words
Shanxian granule/containing serum/epithelial mesenchymal transition/malignant transformation/precancerous lesions of liver/hepatic oval cells/Wnt/β-catenin signaling pathway分类
医药卫生引用本文复制引用
屈梦扬,潘艳芳,贾晓涛,杨雅倩,杨鑫茂,王院春,齐宝宁..山仙颗粒含药血清通过Wnt/β-catenin信号通路抑制肝卵圆细胞EMT及恶性转化相关表型[J].中西医结合肝病杂志,2026,36(4):451-455,5.基金项目
国家自然科学基金项目(No.81703842),陕西省重点研发计划项目(No.2024SF-YBXM-147),咸阳市重点研发计划(No.L2025-ZDYF-JBFZ-017) （No.81703842）
