安徽农学通报2026，Vol.32Issue(10)：35-38,4.DOI:10.16377/j.cnki.issn1007-7731.2026.10.010
基于线粒体16S rRNA基因序列的3个雌核发育锦鲤品系的遗传差异分析
Genetic diversity analysis of three gynogenetic Cyprinus carpio based on mitochondrial 16S rRNA gene sequences
摘要
Abstract
Based on the mitochondrial 16S rRNA gene sequence,this study analyzed the genetic diversity of 3 Cyprinus carpio varieties(pure red,pure white,and pure black)cultivated by gynogenesis using the GenBank database and MEGA 5.0 software.The results showed that the length of PCR amplification products ranged from 750 to 1 000 bp,which met the requirements for sequencing.Sequencing analysis indicated that the length of the extracted 16S rRNA gene fragment was 967 bp,with an average A+T content of 58.26%,which was higher than the G+C content(41.74%),consistent with the A and T bias characteristics of fish mitochondrial genes.Phylogenetic analysis showed that the genetic distances between Cyprinus carpio Gyno Red and Cyprinus carpio Gyno Black,Cyprinus carpio Gyno Black and Cyprinus carpio Gyno White,and Cyprinus carpio Gyno White and Cyprinus carpio Gyno Red were 0.10%,4.80%,and 5.00%,respectively;Cyprinus carpio Gyno Black and Cyprinus carpio Gyno Red had a close genetic relationship,while Cyprinus carpio Gyno White clustered independently into one branch.The research results provide references for the directional selection,genetic improvement of high quality body color strains and population evolution research of Cyprinus carpio.关键词
锦鲤/线粒体/16S rRNA基因/系统发育树/遗传多样性Key words
Cyprinus carpio/mitochondrial/16S rRNA gene/phylogenetic tree/genetic diversity分类
生物科学引用本文复制引用
周智慧,周一帆,黄忆嫚,曹馨雨,龚丁斌..基于线粒体16S rRNA基因序列的3个雌核发育锦鲤品系的遗传差异分析[J].安徽农学通报,2026,32(10):35-38,4.基金项目
国家自然科学基金青年科学基金项目(32302988) （32302988）
水生生物保护与水生态修复安徽省高校工程技术研究中心开放课题(Ss202403) （Ss202403）
安庆师范大学省级大学生创新创业训练计划资助项目(S202410372033). （S202410372033）
