安徽农业科学2026，Vol.54Issue(9)：1-5,5.DOI:10.3969/j.issn.0517-6611.2026.09.001
低温高分子糖降解菌的筛选及产酶条件研究
Screening of Low Temperature Polymeric Sugar Degrading Bacteria and Study on Enzyme Production Conditions
摘要
Abstract
A low temperature bacterium producing amylase and cellulase was isolated and screened from agricultural soil in northern China.After 16S rDNA sequence alignment analysis,it was determined that the bacterium is Glutamicibacter arilaitensis.Preliminary study was con-ducted on the cultivation conditions for producing amylase and cellulase by this bacterium.The results showed that the optimal temperature for the production of two enzymes by this bacterium was 10℃and the optimal pH was 7.After 48 hours of cultivation,the highest amylase activi-ty was observed in the crude enzyme solution,which was 12.744 U/mL.While the optimal fermentation time for producing cellulase was 96 hours,with a highest enzyme activity of 4.882 U/mL.Due to its ability to produce both amylase and cellulase under low temperature condi-tions of 10℃,this strain has certain research value and application prospects in the treatment of agricultural and livestock waste and wastewater in northern regions.关键词
低温/高分子糖降解菌/淀粉酶/纤维素酶/培养条件Key words
Low temperature/Polymeric sugar degrading bacteria/Amylase/Cellulase/Cultivation conditions分类
资源环境引用本文复制引用
张婕,徐春光,乌日力嘎..低温高分子糖降解菌的筛选及产酶条件研究[J].安徽农业科学,2026,54(9):1-5,5.基金项目
2021年度呼伦贝尔学院校级自然科学重点项目(2021ZK-ZD02) （2021ZK-ZD02）
2021年度呼伦贝尔学院校级服务地方重点项目(2021FDZD05). （2021FDZD05）
