生物资源2026，Vol.48Issue(2)：167-178,12.DOI:10.14188/j.ajsh.20260301001
大齿牛果藤中CHS基因家族鉴定与分析
Identification and analysis of the CHS gene family in Nekemias grossedentata
摘要
Abstract
Nekemias grossedentata,the original plant of vine tea,is rich in flavonoids,in which chalcone synthase (CHS) acts as the first key rate-limiting enzyme in the biosynthetic pathway. Based on genome and transcriptome data,this study systematically identified and comprehensively analyzed the genome-wide CHS gene family for the first time. The results revealed a total of 28 CHS members (NgCHS),which were unevenly distributed across seven chromo-somes,with evident tandem duplication events,suggesting that tandem duplication serves as the primary driving force for NgCHS family expansion. Phylogenetic analysis classified the CHS genes into four subgroups,with NgCHS2 and NgCHS7 clustering together with AtCHS from Arabidopsis thaliana,suggesting that they are candidate functional CHS genes. Conserved motif and gene structure analysis confirmed that the family was highly conserved. Cis-acting element analysis showed that NgCHS may be involved in light response,hormone regulation and abiotic stress response. Tissue expression profiling analysis revealed that NgCHS2 and NgCHS7 were highly expressed in young leaves,which is consis-tent with the results of phylogenetic analysis. Enzyme activity assay demonstrated that NgCHS7 catalyzes the condensa-tion of p-coumaroyl-CoA and malonyl-CoA to produce naringenin chalcone,exhibiting typical chalcone synthase activity. This study systematically reveals,for the first time,the member composition,evolutionary characteristics,and enzy-matic function of the CHS gene family in N.grossedentata.These findings provide key genetic resources and a theoretical basis for deciphering the regulatory mechanisms of flavonoid metabolism and for the molecular breeding of high-flavonoid vine tea.关键词
藤茶/大齿牛果藤/查尔酮合酶/基因家族分析/黄酮合成途径Key words
vine tea/Nekemias grossedentata/chalcone synthase/gene family analysis/flavonoid biosynthesis pathway分类
生物科学引用本文复制引用
王若彤,杜瑞豪,沈晓凤,孙超,罗红梅,李滢..大齿牛果藤中CHS基因家族鉴定与分析[J].生物资源,2026,48(2):167-178,12.基金项目
名贵中药资源可持续利用能力建设项目(2060302) （2060302）
北京市自然科学基金(7254483) （7254483）
