生物资源2026，Vol.48Issue(2)：188-195,8.DOI:10.14188/j.ajsh.20260112001
不同产地生地黄主要成分与抗氧化活性评价
Comparative analysis of main components and in vitro antioxidant activities of Rehmannia glutinosa Libosch.from different habitats
摘要
Abstract
To investigate the differences in main chemical components and in vitro antioxidant activity of Rehmannia glutinosa Libosch.from different geographical origins,and to provide a basis for the quality evaluation and rational utiliza-tion of its medicinal resources.The contents of catalpol,rehmannioside D,verbascoside,and echinacoside in 16 batches of Rehmannia glutinosa Libosch.were determined by HPLC-PDA.The antioxidant activities were evaluated by DPPH and ABTS free radical scavenging assays.The relationship between chemical components and antioxidant activity was ex-plored by combining cluster analysis(HCA),principal component analysis(PCA),and Pearson correlation analysis.Re-sults showed that there were significant differences in the chemical composition of samples from different origins.The highest content of catalpol(31.021 mg/g)was found in samples introduced from Linfen,Shanxi to Xinjiang.The high-est content of rehmannioside D(5.633 mg/g)was in samples from Jiaozuo,Henan.The highest contents of echinacoside(0.509 mg/g)and verbascoside(1.352 mg/g)were in samples introduced from Quwo County and Baoyan,Shanxi to Xinjiang,respectively.Samples introduced from Quwo County,Shanxi to Xinjiang exhibited the strongest antioxidant ac-tivity(DPPH IC50 8.97 mg/mL,ABTS IC50 5.29 mg/mL).Correlation analysis indicated that the content of echinaco-side was significantly negatively correlated with the DPPH IC50 value(P<0.05),suggesting it as a key antioxidant com-ponent.In summary,the introduced Rehmannia glutinosa Libosch.in Xinjiang exhibits unique advantages in terms of specific chemical component accumulation and comprehensive antioxidant activity.This study provides a scientific basis for the quality evaluation,origin selection,and identification of key quality markers of Rehmannia glutinosa Libosch.关键词
生地黄/产地/含量测定/抗氧化活性/化学计量学/谱效关系Key words
Rehmannia glutinosa Libosch./geographical origin/content determination/antioxidant activity/chemometrics/spectrum-effect relationship分类
医药卫生引用本文复制引用
夏提古丽·阿不利孜,那非沙·哈力买买提,马丽云,段燕燕,朱军..不同产地生地黄主要成分与抗氧化活性评价[J].生物资源,2026,48(2):188-195,8.基金项目
兵团科技计划(2023AB018-04) （2023AB018-04）
"天山英才"高层次人才培养计划(TSYC202401B177) （TSYC202401B177）
新疆维吾尔自治区"两区"项目(2025LQ03018) （2025LQ03018）
