北华大学学报（自然科学版）2026，Vol.27Issue(2)：204-209,6.DOI:10.11713/j.issn.1009-4822.2026.02.005
五味子酚对小鼠肝癌H22细胞移植瘤生长的影响
Effect of Schisanhenol on the Growth of Transplanted H22 Hepatoma Cells in Mice
摘要
Abstract
Objective To investigate the effect of schisanhenol(SAL)on the growth of transplanted H22 hepa-toma cells in mice.Methods A mouse model of transplanted H22 hepatoma cells was established.After the formation of transplanted tumors,15 C57BL/6 mice were divided into model group,SAL 10 mg/kg group,and SAL 20 mg/kg group,with 5 mice in each group.The volume of transplanted tumors in mice was measured using a vernier caliper,and the tumor inhibition rate was calculated.Hematoxylin-eosin staining and immunohistochemical staining were used to detect the effects of SAL on the histomorphology of transplanted H22 hepatoma tissues and the expression of Ki-67 protein in transplanted tumor tissues,respectively.Western blot was employed to determine the expression levels of p-STAT3(Tyr705),Bcl-2,and Cyclin D1 proteins in mice transplanted tumor tissues.Results Compared with model group,both SAL 10 and 20 mg/kg groups significantly inhibited the volume of transplanted H22 hepatoma cells in mice(P<0.05)in a dose-dependent manner.The results of immunohisto-chemical staining showed that compared with model group,both SAL 10 and 20 mg/kg groups could inhibit the expression of Ki-67 protein in the transplanted H22 hepatoma tissues of mice.Western blot results demonstrated that compared with model group,both SAL 10 and 20 mg/kg groups significantly inhibited the expression of p-STAT3(Tyr705),Bcl-2,and Cyclin D1 proteins(P<0.01)in a dose-dependent manner.Conclusion Schisan-henol inhibits the growth of transplanted H22 hepatoma cells in mice,and its mechanism may be related to the in-hibition of p-STAT3(Tyr705)and the expression of downstream apoptotic gene Bcl-2,as well as the promotion of Cyclin D1 expression.关键词
五味子酚/肝癌/移植瘤生长/p-STAT3(Tyr705)/Cyclin D1Key words
schisanhenol/hepatoma/growth of transplanted tumors/p-STAT3(Tyr705)/Cyclin D1分类
医药卫生引用本文复制引用
吴吉平,张志宏,王玉涵,黄蕾,胡雪阳,李梓晴,李思颖,王春梅,李贺,孙靖辉..五味子酚对小鼠肝癌H22细胞移植瘤生长的影响[J].北华大学学报（自然科学版）,2026,27(2):204-209,6.基金项目
国家自然科学基金青年基金项目(82204697) （82204697）
吉林省自然科学基金项目(YDZJ202201ZYTS275) （YDZJ202201ZYTS275）
北华大学研究生创新计划项目(2023025). （2023025）
