北华大学学报（自然科学版）2026，Vol.27Issue(2)：218-224,7.DOI:10.11713/j.issn.1009-4822.2026.02.007
灵芝外泌体样纳米囊泡对乳腺癌细胞增殖、侵袭及迁移的影响
Effect of Ganoderma lucidum Exosome-like Nanovesicles on Proliferation,Invasion and Migration of Breast Cancer Cells
摘要
Abstract
Objective To investigate the effect of Ganoderma lucidum exosome-like nanovesicles(GLEVs)on proliferation,invasion,and migration of breast cancer(BC)MCF-7 cells,and to clarify the underlying mechanism by which GLEVs inhibit MCF-7 cell functions.Methods GLEVs were isolated and purified from the supernatant of fresh Ganoderma lucidum using a commercial kit,and the morphology of GLEVs was observed by transmission electron microscopy.MCF-7 cells were treated with 40,80,and 120 μg/mL GLEVs for 24 and 48 h,respectively,and the uptake of GLEVs by MCF-7 cells was detected using the DiO dye phagocytosis assay.MCF-7 cells were divided into blank control group,paclitaxel-positive control group,and GLEVs groups at different concentrations(40,80,120 μg/mL).After 24 and 48 h of culture,the cell proliferation capacity of each group was measured by the MTT assay,and the invasion capacity of MCF-7 cells was detected by the Transwell chamber assay.The migra-tion capacity of MCF-7 cells was evaluated by the Transwell chamber assay after 24 h of culture.Results Under transmission electron microscopy,GLEVs exhibited a typical cup-shaped morphology,indicating successful extraction of GLEVs.DiO staining showed that after 24 h of culture,the uptake of 40,80,and 120 μg/mL GLEVs by MCF-7 cells was higher than that in blank control group,and the uptake gradually increased with rising concentrations.After 48 h of culture,GLEVs at various concentrations were effectively internalized by MCF-7 cells.Notably,at a mass concentration of 120 μg/mL,green fluorescence was uniformly distributed around the cell membrane,exhibiting a concentration-dependent manner.MTT assay results demonstrated that compared with blank control group,after 24 h of culture,the cell proliferation in positive control group was significantly reduced(P<0.001),and that in 40 and 120 μg/mL GLEVs groups was also reduced(P<0.05).Compared with blank control group,after 48 h of culture,the cell proliferation in positive control group was significantly reduced(P<0.000 1),that in 40 and 120 μg/mL GLEVs groups was reduced(P<0.01),and that in 80 μg/mL GLEVs group was significantly reduced(P<0.001).Furthermore,the inhibitory effect on cell proliferation gradually weakened with increasing GLEVs concentration.Cell invasion capacity was evaluated by Transwell assay.Com-pared with blank control group,after 24 h of culture,the invasion capacity of 40,80 and 120 μg/mL GLEVs groups was significantly reduced(P<0.01,P<0.05).Transwell assay results indicated that compared with blank control group,80 μg/mL GLEVs group could significantly inhibit the migration capacity of MCF-7 cells(P<0.05).Conclusion GLEVs can be internalized by MCF-7 cells and inhibit the proliferation,invasion,and migration of MCF-7 cells.关键词
灵芝/外泌体样纳米囊泡/乳腺癌/细胞增殖/细胞侵袭/细胞迁移Key words
Ganoderma lucidum/exosome-like nanovesicles/breast cancer/cell proliferation/cell invasion/cell mi-gration分类
医药卫生引用本文复制引用
杨挺,孙一鸣,高艺函,杨婉晴,庄雅东,郝巍..灵芝外泌体样纳米囊泡对乳腺癌细胞增殖、侵袭及迁移的影响[J].北华大学学报（自然科学版）,2026,27(2):218-224,7.基金项目
吉林省教育厅科学技术研究项目(JJKH20240094KJ,JJKH20250820KJ) （JJKH20240094KJ,JJKH20250820KJ）
吉林省科技发展计划项目(20240602082RC,YDZJ202501ZYTS716,20220402072GH). （20240602082RC,YDZJ202501ZYTS716,20220402072GH）
