北华大学学报（自然科学版）2026，Vol.27Issue(2)：225-231,7.DOI:10.11713/j.issn.1009-4822.2026.02.008
红景天苷抑制肝细胞焦亡改善肝纤维化的作用机制
Mechanism of Salidroside in Improving Hepatic Fibrosis by Inhibiting Hepatocyte Pyroptosis
摘要
Abstract
Objective Salidroside(SAL),the main active component of Rhodiola rosea L.,was investigated in this study to deeply analyze the mechanism by which SAL improved hepatic fibrosis by inhibiting hepatocyte pyroptosis.Methods AML-12 cells were induced with lipopolysaccharide(LPS)combined with adenosine triphosphate(ATP)for 4 h,followed by treatment with different concentrations of SAL for 24 h.The protein expressions of NLRP3,ASC,cleaved caspase 1 and GSDMD were detected by Western blot.The mRNA levels of Nlrp3,Casp1 and Il1 b in cells were determined by RT-qPCR.The levels of inflammatory factors IL-6 and IL-1β in hepatocyte supernatants were analyzed by ELISA.Mouse primary hepatic stellate cells(HSCs)were treated with the conditioned medium from mouse primary hepatocytes stimulated by LPS+ATP.After SAL administration,the protein expressions of α-SMA,P2X7R,NLRP3,cleaved caspase 1,IL-23 and mature-IL-1β were detected by Western blot.Results Compared with normal group,LPS+ATP successfully induced AML-12 cell injury and established a hepatocyte pyroptosis model.Western blot results showed that SAL treatment significantly reduced the protein expressions of NLRP3,ASC,cleaved caspase 1 and GSDMD(P<0.01,P<0.001).RT-qPCR results indicated that SAL significantly decreased the mRNA levels of Nlrp3,Casp1 and Il1 b in AML-12 cells(P<0.001).ELISA results demonstrated that SAL could significantly inhibit the extracellular release of IL-1β and IL-6(P<0.001).The experiment also found that the conditioned medium from mouse primary hepatocytes stimulated by LPS+ATP could successfully induce the activation of mouse primary HSCs,and SAL treatment significantly reduced the protein expressions of α-SMA,P2X7R,NLRP3,cleaved caspase 1,IL-23 and mature-IL-1β in HSCs(P<0.001).Conclusion SAL can effectively downregulate the expression of inflammatory factors in hepatocytes,inhibit cell pyroptosis,and regulate the interaction between hepatocytes and hepatic stellate cells.These results suggest that SAL may serve as a potential candidate drug for anti-hepatic fibrosis.关键词
红景天苷/肝纤维化/细胞焦亡/炎症Key words
salidroside/liver fibrosis/pyroptosis/inflammation分类
医药卫生引用本文复制引用
王万灵,刘官成,姜禹辰,孙海明,宋健..红景天苷抑制肝细胞焦亡改善肝纤维化的作用机制[J].北华大学学报（自然科学版）,2026,27(2):225-231,7.基金项目
国家自然科学基金项目(82304848). （82304848）
