草业学报2026，Vol.35Issue(5)：99-112,14.DOI:10.11686/cyxb2025235
三江源区燕麦与箭筈豌豆混播对根际土壤微生物多样性的影响
Effects of mixed sowing of oat and common vetch on the microbial diversity of inter-root soil in the Sanjiangyuan region
摘要
Abstract
The Sanjiangyuan region is an important ecological barrier in China,and the diversity of soil microorganisms in this region is critical for sustaining ecosystem stability.This study created artificial mixed sowings of oat(Avena sativa)and common vetch(Vicia sativa)at ratios of 7∶3(Y7J3),5∶5(Y5J5),and 3∶7(Y3J7),with oat monoculture and common vetch monoculture as controls,in order to examine the effects of mixed sowing on soil nutrients and rhizosphere microbial communities.The Mantel test was used to assess the connections between changes in soil nutrients,rhizosphere microbial community structure,and diversity.It was found that mixed sowing considerably raised the soil pH,organic matter,total nitrogen,and available phosphorus content(P<0.05).The Y7J3 treatment showed the greatest improvement(P<0.05).This sowing mix considerably enhanced the relative abundance of Acidobacteriota,RB41 and Pseudomonas in the bacterial community and Ascomycota,Mortierellomycota,Thelebolus and Exophiala in the fungal community(P<0.05).Mantel test analysis revealed that the main elements influencing the control of soil microbial communities were soil pH,organic matter,available nitrogen,and available phosphorus(P<0.05).In conclusion,by altering the inter-root soil nutrients,the mixed sowing of oat and common vetch had a significant impact on the structure and diversity of the soil microbial community.The best results were obtained when the oat and common vetch were sown in 7∶3,and soil pH and organic matter were highly important in the regulation of the microbial community.These results provide a scientific basis for useful recommendations for enhancing soil ecological function and optimizing artificial grassland planting in the Sanjiangyuan region.关键词
燕麦/箭筈豌豆/混播比例/高通量测序/土壤养分/根际微生物Key words
oat/common vetch/mixed planting ratio/high-throughput sequencing/soil nutrients/rhizosphere microbiology引用本文复制引用
李菲,张琳,德科加,冯廷旭,林伟山,向雪梅,魏希杰..三江源区燕麦与箭筈豌豆混播对根际土壤微生物多样性的影响[J].草业学报,2026,35(5):99-112,14.基金项目
青海省科技厅重点研发与转化计划项目(2024-NK-137),国家重点研发计划课题(2022YFD1602302)和青海大学三江源生态系统教育部野外科学观测研究站资助. （2024-NK-137）
