草地学报2026，Vol.34Issue(5)：1597-1609,13.DOI:10.11733/j.issn.1007-0435.2026.05.004
沙葱萤叶甲幼虫对蜕皮激素响应的转录组学分析
Transcriptome Analysis of Galeruca daurica Larval Response to 20-Hydroxyecdysone
摘要
Abstract
Larvae of Galeruca daurica are the major pest that severely damages the grasslands of Inner Mongo-lia.To explore the molecular mechanisms by which 20-hydroxyecdysone(20E)regulates the development of its larval,transcriptome sequencing was conducted on 3rd-instar larvae treated with 20E for 48 hours by using the Illumina HiSeqTM 4000 platform.A total of 75 536 Unigenes were obtained,among which 40 161 were functionally annotated.Compared with the control group,a total of 500 differentially expressed genes(DEGs)were identified,including 258 upregulated and 242 downregulated ones.GO and KEGG analyses revealed that the DEGs were significantly enriched in terms/pathways such as ion transport,catalytic activity,longevity regu-lating pathway-multiple species,and FOXO signaling pathway.Furthermore,11 ecdysone-related genes were screened out.The qRT-PCR verification results confirmed the reliability of the sequencing data.The results indicated that 20E may regulate the growth and development of G.daurica larvae by affecting multiple meta-bolic pathways.This study systematically analyzed the transcriptomic characteristics of G.daurica larvae in response to 20E regulation,providing a theoretical basis for clarifying its developmental mechanism and screen-ing green control targets.关键词
沙葱萤叶甲/幼虫/蜕皮激素/转录组分析/蜕皮相关基因/FOXO信号通路Key words
Galeruca daurica/Larvae/20-hydroxyecdysone/Transcriptome analysis/Ecdysis-related genes/FOXO signaling pathway分类
农业科技引用本文复制引用
刘利,高丽,琪勒莫格,郭宁,邢颖,李晓海,段天凤..沙葱萤叶甲幼虫对蜕皮激素响应的转录组学分析[J].草地学报,2026,34(5):1597-1609,13.基金项目
内蒙古自治区自然科学基金(2023QN03059) （2023QN03059）
内蒙古自治区高等学校科学研究项目(NJZZ23109) （NJZZ23109）
包头师范学院高层次人才引进科研启动基金(BTTCRCQD2023-013) （BTTCRCQD2023-013）
内蒙古自治区高校科技研究项目(NJZY23005) （NJZY23005）
包头师范学院高水平研究成果培育自然科学类青年项目(BSYKJ2023-ZQ02)资助 （BSYKJ2023-ZQ02）
