南方医科大学学报2026，Vol.46Issue(5)：1006-1017,12.DOI:10.12122/j.issn.1673-4254.2026.05.04
薤白超细粉通过抑制铁死亡并调控NF-κB/STAT3信号轴缓解脂多糖诱导的小鼠急性肺损伤
Ultrafine Xiebai powder alleviates lipopolysaccharide-induced acute lung injury in mice by suppressing ferroptosis and modulating the NF-κB/STAT3 signaling axis
摘要
Abstract
Objective To investigate the protective effects of ultrafine Xiebai powder(UXP)against lipopolysaccharide(LPS)-induced acute lung injury(ALI)in mice and the underlying mechanism.Methods Fifty-four male C57BL/6 mice were randomized into 9 groups,including a control group and 8 intratracheal LPS instillation-induced ALI model groups gavaged with saline,dexamethasone(DEX,1 mg/kg),or 100,250,or 500 mg/kg UXP or granule Xiebai powder(n=6)for 6 consecutive days,starting on the day of modeling.After the treatment,the lung wet-to-dry(W/D)ratios were determined,inflammatory cytokines in bronchoalveolar lavage fluid(BALF)were measured by ELISA,and inflammatory cell infiltration was assessed by flow cytometry.Histopathological injury of the lungs was observed using HE staining,and pulmonary SOD,MDA,and GSH levels were determined.Pulmonary mRNA expressions of inflammatory mediators were quantified by RT-qPCR,and NF-κB and STAT3 activation was analyzed using Western blotting;mitochondrial ultrastructure was examined with transmission electron microscopy.In a LPS-stimulated BEAS-2B cell model,the effects of UXP and DEX were assessed on cell viability,apoptosis,reactive oxygen species(ROS),mitochondrial function,lipid peroxidation,and ferrous iron levels.Results The LPS-challenged mice exhibited a higher lung W/D ratio and increased BALF cytokine levels and inflammatory cell counts with pronounced lung inflammation and tissue damage,lowered SOD and GSH levels,elevated MDA levels,increased mRNA expressions of inflammatory mediators and the p-NF-κB/NF-κB and p-STAT3/STAT3 ratios,and obvious mitochondrial damages.UXP markedly alleviated these changes with an efficacy comparable to DEX.In LPS-stimulated BEAS-2B cells,UXP significantly improved cell viability,redcued cell apoptosis and ROS accumulation,preserved mitochondrial integrity,and reduced lipid peroxidation and ferroptosis-associated changes.Conclusion UXP has strong protective effects against LPS-induced ALI in mice possibly by suppressing inflammation,oxidative stress,ferroptosis,and apoptosis,modulating the NF-κB/STAT3 signaling axis,and maintaining mitochondrial integrity.关键词
薤白超细粉/急性肺损伤/炎症/氧化应激/铁死亡Key words
ultrafine Xiebai powder/acute lung injury/inflammation/oxidative stress/ferroptosis引用本文复制引用
祝金超,邵宁宁,黄泽彧,刘燕青,孟祥艳,刘子泉,孙予杰,董津睿,樊毫军..薤白超细粉通过抑制铁死亡并调控NF-κB/STAT3信号轴缓解脂多糖诱导的小鼠急性肺损伤[J].南方医科大学学报,2026,46(5):1006-1017,12.基金项目
国家科技部重点研发计划项目(2024YFC3016604) （2024YFC3016604）
国家应急管理部重点实验室自主创新基金项目(YJBZZJJTJU202403) （YJBZZJJTJU202403）
国家自然科学基金(82200655) （82200655）
国家级大学生创新训练计划项目(202310056123)Supported by National Natural Science Foundation of China(82200655). （202310056123）
