南方医科大学学报2026，Vol.46Issue(5)：1059-1065,7.DOI:10.12122/j.issn.1673-4254.2026.05.09
优化复合应激方案成功构建模拟临床病因的胃功能障碍与抑郁共病模型
Establishment of an gastric dysfunction and depression comorbidity model simulating clinical etiology in mice using a combined stress protocol
摘要
Abstract
Objective To establish a mouse model with the comorbidity of gastric dysfunction and depression mimicking clinical onset of the condition.Methods Thirty male C57BL/6J mice were randomly divided into control,14-day model,and 28-day model groups(n=10).The mice in the latter two groups were subjected to continuous combined stresses induced by tail clamping,cold-saline gavage and irregular feeding for 14 or 28 days.Gastric motility,gastric emptying and histopathological changes of the mice were evaluated using strain gauge sensors,in vivo imaging and HE staining,respectively,and behavioral tests were used to assess their depression-like behaviors.Serum corticotropin-releasing factor(CRF),brain-derived neurotrophic factor(BDNF),gastrin,motilin,and gastric nitric oxide synthase(NOS)and acetylcholinesterase(AChE)levels were measured by ELISA to explore the peripheral mechanisms.Results Compared with control mice,the mice in 14-day model group exhibited reduced body weight,food intake and gastric motility amplitude with delayed gastric emptying but showed no significant depression-like behaviors;in 28-day model group,these changes were further exacerbated,and significant depression-like behaviors were observed.Compared with 14-day model group,the 28-day model group displayed severer gastric dysfunction and depression-like behaviors.Compared with control group,exposure to complex stresses for 14 days significantly increased serum CRF and decreased BDNF,gastrin and motilin levels,increased gastric NOS and decreased gastric AChE level,which were worsened after a 28-day exposure.HE staining showed no obvious histological changes in the gastric mucosa in the two model groups.Conclusion Combined stresses induced by tail clamping,cold-saline gavage and irregular feeding for 28 days can induced the comorbidity of gastric dysfunction and depression in mice,and abnormal changes in serum neurotransmitters,brain-gut peptides and vagus activity may partly participate in the pathogenesis of the comorbidity.关键词
胃功能障碍/抑郁情绪/动物模型/复合应激/胃动力/行为学评价Key words
gastric dysfunction/depression-like behavior/animal model/combined stress/gastric motility/behavioral assessment引用本文复制引用
黄清扬,沈雯雯,胡进,马宏坤,胡雁冰,张程杰,申国明,王浩..优化复合应激方案成功构建模拟临床病因的胃功能障碍与抑郁共病模型[J].南方医科大学学报,2026,46(5):1059-1065,7.基金项目
安徽省自然科学基金(2408085MH223) （2408085MH223）
安徽省高校优秀青年科研项目(2022AH030065) （2022AH030065）
国家自然科学基金(82474224) （82474224）
经脉脏腑相关安徽省重点实验室开放课题(2024AHMVC04)Supported by National Natural Science Foundation of China(82474224). （2024AHMVC04）
