中国耳鼻咽喉头颈外科2026，Vol.33Issue(2)：87-92,6.DOI:10.16066/j.1672-7002.2026.02.006
pGlu-Apelin-13酰胺通过调节耳蜗巨噬细胞极化及抑制NLRP3炎症小体活化缓解噪声性听力损失的机制研究
Research on the mechanism by which pGlu-Apelin-13 amide alleviates noise-induced hearing loss by regulating cochlear macrophage polarization and inhibiting NLRP3 inflammasome activation
摘要
Abstract
OBJECTIVE To investigate the intervention effect and mechanism of pGlu-Apelin-13 amide on noise-induced hearing loss in mice.METHODS Forty C57BL/6J mice were randomly divided into a control group,a model group,and low-,medium-,and high-dose pGlu-Apelin-13 amide groups,with eight mice in each group.The auditory brainstem response(ABR)threshold was measured.Cochlear morphology was observed by HE staining.The mRNA expression of inflammatory factors IL-10,IL-6,TNF-α,and Arg-1 was detected by RT-qPCR.The polarization of M1/M2 macrophages in the cochlear basilar membrane was detected by immunofluorescence.Serum IL-1β and IL-18 levels were detected by ELISA.The protein expression of NLRP3,ASC,and Caspase-1 was detected by immunohistochemistry and Western blot.RESULTS Compared with the model group,mice in the low,medium,and high dose pGlu-Apelin-13 amide groups showed decreased ABR thresholds at all frequencies,and reduced damage to cochlear hair cells and spiral ganglia;IL-10 and Arg-1 expression was upregulated,while IL-6,TNF-α,IL-1β,and IL-18 levels were decreased,the number of M1 macrophages and the number of M2 macrophages in the cochlear basilar membrane were reduced;NLRP3,ASC,and Caspase-1 protein expression were all significantly downregulated(P<0.05).Except for the ABR threshold,all other indicators showed significant dose-dependency(P<0.05).CONCLUSION pGlu-Apelin-13 amide can alleviate noise-induced hearing loss in mice,and its mechanism is related to regulating macrophage phenotypic polarization and inhibiting NLRP3 inflammasome activation.关键词
听觉丧失,噪声性/巨噬细胞/pGlu-Apelin-13酰胺/巨噬细胞极化/NLR家族Pyrin域蛋白3炎症小体Key words
Hearing Loss,Noise-Induced/Macrophages/pGlu-Apelin-13 amide/macrophage polarization/NLR family pyrin domain protein 3 inflammasome引用本文复制引用
周彩玲,乐燕娜,胡祖应,李勇,朱赛群..pGlu-Apelin-13酰胺通过调节耳蜗巨噬细胞极化及抑制NLRP3炎症小体活化缓解噪声性听力损失的机制研究[J].中国耳鼻咽喉头颈外科,2026,33(2):87-92,6.基金项目
浙江省医药卫生科技项目(2025KY171) （2025KY171）
浙江省疾病预防控制科技计划项目:(2026JKY191) （2026JKY191）
