福建农业科技2026，Vol.57Issue(4)：19-28,10.DOI:10.13651/j.cnki.fjnykj.2026.04.003
基于LC-Q/TOF-MS技术分析不同芽胞杆菌产surfactin的多态性特征
Analysis of Surfactin Polymorphism Produced by Different Bacillus Strains Based on LC-Q/TOF-MS
摘要
Abstract
Surfactin is a class of lipopeptide compounds produced by Bacillus species with significant biocontrol potential.The diversity of its structure and composition directly affects its biological activity.In this study,the liquid chromatography-quadrupole time-of-flight mass spectrometry(LC-Q/TOF-MS)was employed to analyze the compound composition,carbon chain distribution,derivative characteristics,and yield differences of surfactin produced by 18 strains of Bacillus(including closely related genera),aiming to reveal its chemical polymorphism.The results showed that the tested strains could be classified into high-abundance type(≥7 compounds),medium-abundance type(5-6 compounds),and low-abundance type(≤4 compounds)based on the number of surfactin compounds.The carbon chain distribution revealed that C14 and C15 were common to all strains;C13 was present in 11 strains(absent in the remaining 7),while C12 was detected in only two strains;and the long-chain compounds with C16 and above were present only in some strains.The distribution of surfactin derivatives also exhibited strain specificity,with the C15 surfactin derivatives showing the broadest distribution,and only the two strains(Bacillus licheniformis FJAT-8771 and Bacillus alcalophilus FJAT-10014)completely lacking all derivatives.The yield analysis indicated that Bacillus subtilis subsp.inaquosorum FJAT-14251 and Bacillus firmus FJAT-8764 were high-yielding strains,whereas Alkalibacillus haloalkaliphilus FJAT-10023 was a low-yielding strain.The further correlation analysis showed that surfactin production was positively correlated with its compound polymorphism.The high-yield strains generally had the characteristics of high abundance(≥7 compounds),broad carbon chain range(including long chains with C16 and above)and multiple derivatives(≥3 compounds).This study provided data support for understanding the metabolic diversity in Bacillus,and also provided a reference for screening the superior strains with high production of specific surfactin compounds and for developing the efficient and stable biological pesticides.关键词
芽胞杆菌/脂肽/表面活性素/液相色谱-四极杆飞行时间质谱/多态性Key words
Bacillus/Lipopeptide/Surfactin/Liquid chromatography-quadrupole time-of-flight mass spectrometry/Polymorphism分类
资源环境引用本文复制引用
陈峥,林恩泉,谢东俊,杨显英,刘波,陈梅春..基于LC-Q/TOF-MS技术分析不同芽胞杆菌产surfactin的多态性特征[J].福建农业科技,2026,57(4):19-28,10.基金项目
福建省自然科学基金项目(2023J01378). （2023J01378）
