福建师范大学学报（自然科学版）2026，Vol.42Issue(3)：45-52,8.DOI:10.12046/j.issn.1000-5277.2025120037
长期氮磷施肥对黑土氨氧化微生物丰度及硝化潜势的影响
Effects of Long-Term Nitrogen and Phosphorus Fertilization on the Abundance of Ammonia-Oxidizing Microorganisms and Potential Nitrification Rate in Black Soil
摘要
Abstract
This study investigates the effects of long-term fertilization on the abundance of am-monia-oxidizing microorganisms and potential nitrification rate in black soil,based on a long-term(40 years)fertilization experiment in Northeast China.Soil samples were collected from four treat-ments,including no nitrogen fertilizer(CK),nitrogen fertilizer only(N),phosphorus fertilizer only(P),and combined nitrogen-phosphorus fertilizers(NP),over three consecutive years(2021-2023).The abundance of ammonia-oxidizing microorganisms was quantified via real-time quantitative PCR,and the soil potential nitrification rate was measured using the chlorate inhibition method.The results showed that both N and NP treatments significantly decreased the abundance of ammonia-oxi-dizing archaea,comammox Nitrospira Clade B,and the soil potential nitrification rate,while signifi-cantly increasing the abundance of ammonia-oxidizing bacteria,comammox Nitrospira Clade A,and soil NO3--N content.The P treatment had no significant effect on the abundance of ammonia-oxidizing microorganisms,but significantly increased the soil potential nitrification rate.Correlation analysis in-dicated that the abundance of ammonia-oxidizing archaea was the dominant biotic factor influencing the soil potential nitrification rate,while soil pH was the main environmental factor.In conclusion,long-term fertilization regulates soil nitrification in black soil by altering the abundance of ammonia-oxidizing microorganisms and soil pH.关键词
黑土/氨氧化古菌/amoA基因/完全氨氧化菌/长期施肥Key words
black soil/ammonia-oxidizing archaea/amoA gene/comammox/long-term fertili-zation分类
农业科技引用本文复制引用
常钰海,马星竹,刘子恺,陈文锐,王思仪,李娴,沈菊培..长期氮磷施肥对黑土氨氧化微生物丰度及硝化潜势的影响[J].福建师范大学学报（自然科学版）,2026,42(3):45-52,8.基金项目
国家自然科学基金项目(32171642) （32171642）
